| 货号 | 94350S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H M R |
| 来源宿主 | Rabbit IgG |
| 应用 | W |
| 目标/特异性 | NLK (D9X3C) Rabbit mAb recognizes endogenous levels of total NLK protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Nemo-like kinase (NLK ) is a serine/threonine-protein kinase that regulates multiple signaling pathways, including Wnt/β-catenin, TGFβ, IL-6, and Notch (1-4). NLK contributes to cell proliferation, differentiation, cell fate determination during early embryogenesis and nervous system development in vertebrates (5-7). Recent studies showed that NLK is aberrantly expressed in various types of cancer where it regulates cancer cell proliferation, migration, invasion and survival (8-11). NLK is localized predominantly in nucleus and at a lower level in cytoplasm(12). Homodimerization of NLK is required for its activation and nuclear localization. NLK is activated via intermolecular autophosphorylation at Thr286 (13). NLK interacts with and phosphorylates a number of transcription factors including FOXO1, FOXO4, MYB, NOTCH1 and TCF7L2/TCF4, and LEF-1/TCF (14-18). NLK also associates with E3 ubiquitin ligase NARF and Raptor and regulates their function (19,20). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 58 |
| 参考文献 | 1 . Smit, L. et al. (2004) J Biol Chem 279, 17232-40. 2 . Ohkawara, B. et al. (2004) Genes Dev 18, 381-6. 3 . Kojima, H. et al. (2005) Proc Natl Acad Sci U S A 102, 4524-9. 4 . Ishitani, T. et al. (2010) Nat Cell Biol 12, 278-85. 5 . Hyodo-Miura, J. et al. (2002) Genes Cells 7, 487-96. 6 . Thorpe, C.J. and Moon, R.T. (2004) Development 131, 2899-909. 7 . Satoh, K. et al. (2007) Mol Cell Biol 27, 7623-30. 8 . Li, M. et al. (2013) Tumour Biol 34, 3995-4000. 9 . Lv, L. et al. (2014) J Cell Biochem 115, 81-92. 10 . Huang, Y. et al. (2013) PLoS One 8, e69148. 11 . Dong, J.R. et al. (2013) Asian Pac J Cancer Prev 14, 7137-41. 12 . Brott, B.K. et al. (1998) Proc Natl Acad Sci U S A 95, 963-8. 13 . Ishitani, S. et al. (2011) Mol Biol Cell 22, 266-77. 14 . Ishitani, T. et al. (2003) Mol Cell Biol 23, 1379-89. 15 . Kanei-Ishii, C. et al. (2004) Genes Dev 18, 816-29. 16 . Kim, S. et al. (2010) J Biol Chem 285, 8122-9. 17 . Togi, S. et al. (2011) J Biol Chem 286, 19170-7. 18 . Szypowska, A.A. et al. (2011) Antioxid Redox Signal 14, 563-78. 19 . Yamada, M. et al. (2006) J Biol Chem 281, 20749-60. 20 . Yuan, H.X. et al. (2015) Genes Dev 29, 2362-76. |
Western blot analysis of extracts from SW480, HCT 116 and C2C12 cells using NLK (D9X3C) Rabbit mAb. |
