| 货号 | 91894S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H M R Mk |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | PBRM1 (D3F7O) Rabbit mAb recognizes endogenous levels of total PBRM1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin (2-5). The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1 to regulate gene activation, cell growth, the cell cycle, and differentiation processes (1,6-9). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 200 |
| 参考文献 | 1 . Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84. 2 . Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73. 3 . Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11. 4 . Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81. 5 . Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17. 6 . Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32. 7 . Morettini, S. et al. (2008) Front Biosci 13, 5522-32. 8 . Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6. 9 . Simone, C. (2006) J Cell Physiol 207, 309-14. 10 . Nie, Z. et al. (2000) Mol Cell Biol 20, 8879-88. 11 . Xue, Y. et al. (2000) Proc Natl Acad Sci U S A 97, 13015-20. 12 . Lemon, B. et al. Nature 414, 924-8. 13 . Wang, Z. et al. (2004) Genes Dev 18, 3106-16. 14 . Varela, I. et al. (2011) Nature 469, 539-42. 15 . Xia, W. et al. (2008) Cancer Res 68, 1667-74. 16 . Mo, D. et al. (2015) Int J Clin Exp Pathol 8, 9307-13. |
Western blot analysis of extracts from various cell lines using PBRM/BAF180 (D3F7O) Rabbit mAb (upper) or α-Actinin (D6F6) XP® mAb #6487 (lower). | |
Immunoprecipitation of PBRM1/BAF180 from Jurkat cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PBRM1/BAF180 (D3F7O) Rabbit mAb. Western blot analysis was performed using PBRM1/BAF180 (D3F7O) Rabbit mAb. |
