| 货号 | 91735S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H M R Mk |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IHC-P ChIP |
| 目标/特异性 | SMARCB1/BAF47 (D8M1X) Rabbit mAb recognizes endogenous levels of total SMARCB1/BAF47 protein. |
| 使用方法 | WB(1:1000) IP (1:200) IHC-P (1:1000) ChIP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors, such as nuclear receptors, p53, Rb, BRCA1, and MyoD, facilitate recruitment of the complex to target genes for regulation of gene activation, cell growth, cell cycle, and differentiation processes (1,6-9). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 44 |
| 参考文献 | 1 . Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84. 2 . Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73. 3 . Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11. 4 . Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81. 5 . Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17. 6 . Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32. 7 . Morettini, S. et al. (2008) Front Biosci 13, 5522-32. 8 . Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6. 9 . Simone, C. (2006) J Cell Physiol 207, 309-14. 10 . Phelan, M.L. et al. (1999) Mol Cell 3, 247-53. 11 . Versteege, I. et al. (1998) Nature 394, 203-6. 12 . Biegel, J.A. et al. (1999) Cancer Res 59, 74-9. 13 . Klochendler-Yeivin, A. et al. (2000) EMBO Rep 1, 500-6. 14 . Gresh, L. et al. (2005) EMBO J 24, 3313-24. |
Immunoprecipitation of SNF5 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb XP® Isotype Control #3900, and lane 3 is SNF5 (D8M1X) Rabbit mAb. Western blot analysis was performed using SNF5 (D8M1X) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using SNF5 (D8M1X) Rabbit mab (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower). | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using SNF5 (D8M1X) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded HeLa (left) and A204 (right) cell pellets using SNF5 (D8M1X) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse colon using SNF5 (D8M1X) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d followed by treatment with β-estradiol (10 nM, 45 min) and either 10 μl of SNF5 (D8M1X) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human ESR1 Promoter Primers #9673, SimpleChIP® Human pS2 Promoter Primers #9702, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SNF5 (D8M1X) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded endometrioid adenocarcinoma using SNF5 (D8M1X) Rabbit mAb. |
