| 货号 | 90673S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H M |
| 来源宿主 | Rabbit IgG |
| 应用 | W |
| 目标/特异性 | EphA1 (D6V7I) Rabbit mAb recognizes endogenous levels of total EphA1 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands. While EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand, EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer (3). Both ephrin A and B ligands have dual functions. As RTK ligands, ephrins stimulate the kinase activity of Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 130 |
| 参考文献 | 1 . Wilkinson, D.G. (2000) Int Rev Cytol 196, 177-244. 2 . Klein, R. (2001) Curr Opin Cell Biol 13, 196-203. 3 . Dodelet, V.C. and Pasquale, E.B. (2000) Oncogene 19, 5614-9. 4 . Holder, N. and Klein, R. (1999) Development 126, 2033-44. 5 . Brückner, K. et al. (1997) Science 275, 1640-3. 6 . Coulthard, M.G. et al. (2001) Growth Factors 18, 303-17. 7 . Yamazaki, T. et al. (2009) J Cell Sci 122, 243-55. 8 . Naj, A.C. et al. (2011) Nat Genet 43, 436-41. 9 . Hollingworth, P. et al. (2011) Nat Genet 43, 429-35. 10 . Wang, Y. et al. (2016) J Exp Clin Cancer Res 35, 65. 11 . Herath, N.I. et al. (2009) Br J Cancer 100, 1095-102. 12 . Hafner, C. et al. (2006) Mod Pathol 19, 1369-77. 13 . Herath, N.I. et al. (2009) Br J Cancer 100, 1095-102. 14 . Hafner, C. et al. (2006) Mod Pathol 19, 1369-77. 15 . Pasquale, E.B. (2008) Cell 133, 38-52. |
Western blot analysis of extracts from LoVo, HT-29 and mIMCD-3 cell lines using EphA1 (D6V7I) Rabbit mAb. |
