| 货号 | 90540S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | TACSTD2/TROP2 (D1W5W) Rabbit mAb recognizes endogenous levels of total TACSTD2/TROP2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TROP2 is a transmembrane glycoprotein encoded by gene TACSTD2 (tumor associated calcium signal transducer 2). TROP2 was first discovered as a biomarker of invasive trophoblast cells and later reported in many types of cancer cells as well as in various organs during development, and adult stem cells during homeostasis (1, 2). TROP2 has an extracellular domain with EGF thyroglobulin type-1 repeats, a transmembrane domain and a short cytoplasmic tail with a HIKE domain containing a PIP2 binding site and PKC phosphoryaltion site (Ser303) (1-4). TROP2 functions by regulating multiple signaling pathways including-interaction of extracellular domain with integrin beta1 to regulate FAK signaling, association of its transmembrane domain with claudin1 and claudin7 for tight junction formation, as well as regulation of intracellular calcium release by its PIP2 binding and activation of the ERK/MAPK pathway (1,2, 5-8). All of these functions are important for its role in tumor proliferation, metastasis and invasion (1,2). PKC can phosphorylate TROP2 at Ser303; the phosphorylation changes the cytoplasmic tail conformation and further promotes its signaling (9). TROP2 can be activated through intramembrane proteolysis first by TACE, followed further cleavage by presenilin 1 and presenilin2. The proteolysis process is required for its role in tumor cell proliferation (10,11). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 45-65 |
| 参考文献 | 1 . McDougall, A.R. et al. (2015) Dev Dyn 244, 99-109. 2 . Shvartsur, A. and Bonavida, B. (2015) Genes Cancer 6, 84-105. 3 . El Sewedy, T. et al. (1998) Int J Cancer 75, 324-30. 4 . Linnenbach, A.J. et al. (1993) Mol Cell Biol 13, 1507-15. 5 . Trerotola, M. et al. (2013) Cancer Res 73, 3155-67. 6 . Trerotola, M. et al. (2015) Oncotarget 6, 14318-28. 7 . Nakatsukasa, M. et al. (2010) Am J Pathol 177, 1344-55. 8 . Cubas, R. et al. (2010) Mol Cancer 9, 253. 9 . Pavšič, M. et al. (2015) Sci Rep 5, 10324. 10 . Stoyanova, T. et al. (2012) Genes Dev 26, 2271-85. 11 . Ju, X. et al. (2016) Cancer Res , . |
Immunoprecipitation of TACSTD2 protein from UACC-812 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is TACSTD2/TROP2 (D1W5W) Rabbit mAb. Western blot analysis was performed using TA CSTD2/TROP2 (D1W5W) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using TACSTD2/TROP2 (D1W5W) Rabbit mAb. |
