| 货号 | 87809S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H M |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | MMP-2 (D2O4T) Rabbit mAb recognizes endogenous levels of total MMP-2 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors, and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7, and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis, and apoptosis (2-4). Research studies have shown that MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full length protein (5). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 64,72 |
| 参考文献 | 1 . McCawley, L.J. and Matrisian, L.M. (2001) Curr Opin Cell Biol 13, 534-40. 2 . Coussens, L.M. et al. (2002) Science 295, 2387-92. 3 . Sternlicht, M.D. et al. (1999) Cell 98, 137-46. 4 . Vu, T.H. et al. (1998) Cell 93, 411-22. 5 . Nagase, H. et al. (1990) Biochemistry 29, 5783-9. |
Immunoprecipitation of MMP-2 protein from U87 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MMP-2 (D2O4T) Rabbit mAb. Western blot analysis was performed using MMP-2 (D2O4T) Rabbit mAb. | |
Western blot analysis of extracts from U87, 3T3-L1 and C2C12 cells using MMP-2 (D4O4T) Rabbit mAb. |
