| 货号 | 86875S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H |
| 来源宿主 | Rabbit IgG |
| 应用 | W |
| 目标/特异性 | Bok (D7V2N) Rabbit mAb recognizes endogenous levels of total Bok protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Bcl-2 family consists of a number of evolutionarily conserved proteins containing Bcl-2 homology domains (BH) that regulate apoptosis through control of mitochondrial membrane permeability and release of cytochrome c (1-3). Four BH domains have been identified (BH1-4) that mediate protein interactions. The family can be separated into three groups based upon function and sequence homology: pro-survival members include Bcl-2, Bcl-xL, Mcl-1, A1 and Bcl-w; pro-apoptotic proteins include Bax, Bak and Bok, and "BH3 only" proteins Bad, Bik, Bid, Puma, Bim, Bmf, Noxa and Hrk. Interactions between death-promoting and death-suppressing Bcl-2 family members has led to a rheostat model in which the ratio of pro-apoptotic and anti-apoptotic proteins controls cell fate (4). Thus, pro-survival members exert their behavior by binding to and antagonizing death-promoting members. In general, the "BH3-only members" can bind to and antagonize the pro-survival proteins leading to increased apoptosis (5). While some redundancy of this system likely exists, tissue specificity, transcriptional and post-translational regulation of many of these family members can account for distinct physiological roles. |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 22 |
| 参考文献 | 1 . Cory, S. et al. (2003) Oncogene 22, 8590-607. 2 . Antonsson, B. and Martinou, J.C. (2000) Exp Cell Res 256, 50-7. 3 . Sharpe, J.C. et al. (2004) Biochim Biophys Acta 1644, 107-13. 4 . Korsmeyer, S.J. et al. (1993) Semin Cancer Biol 4, 327-32. 5 . Bouillet, P. and Strasser, A. (2002) J Cell Sci 115, 1567-74. 6 . Hsu, S.Y. et al. (1997) Proc Natl Acad Sci U S A 94, 12401-6. 7 . Inohara, N. et al. (1998) J Biol Chem 273, 8705-10. 8 . Beroukhim, R. et al. (2010) Nature 463, 899-905. 9 . Ke, F. et al. (2012) Cell Death Differ 19, 915-25. 10 . Carpio, M.A. et al. (2015) Proc Natl Acad Sci U S A 112, 7201-6. 11 . Llambi, F. et al. (2016) Cell 165, 421-33. |
Western blot analysis of extracts from various cell lines using Bok (D7V2N) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Bok (hBok-Myc/DDK; +) using Bok (D7V2N) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower). |
