| 货号 | 84934S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H |
| 来源宿主 | Rabbit IgG |
| 应用 | W |
| 目标/特异性 | Hemoglobin β (D4W4I) Rabbit mAb recognizes endogenous levels of the hemoglobin β subunit. This antibody may also detect the hemoglobin δ subunit, but is not predicted to cross-react with hemoglobin α, γ, ε or ζ subunits. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Hemoglobin (Hb, Hgb) is a heme-containing transport protein found primarily in the red blood cells of humans and most other vertebrates. The primary function of hemoglobin is to transport oxygen from the external environment to the body tissues. Hemoglobin also facilitates metabolic waste removal by assisting in the transport of carbon dioxide from tissues back to the respiratory organs (1). Mature hemoglobin is a tetrameric protein complex, with each subunit containing an oxygen-binding heme group (2). Multiple isoforms of hemoglobin exist, which vary in relative abundance depending on developmental stage. Adult hemoglobin (HbA) is comprised of two α subunits and two β subunits and is the predominant hemoglobin found in red blood cells of children and adults. Fetal hemoglobin (HbF) contains two α subunits and two γ subunits and is the predominant isoform found during fetal and early postnatal development (2,3). Mutations that alter the structure or abundance of specific globin subunits can result in pathological conditions known as hemoglobinopathies (4). One such disorder is sickle cell disease, which is characterized by structural abnormalities that limit the oxygen carrying capacity of red blood cells. By contrast, thalassemia disorders are characterized by deficiencies in the abundance of specific hemoglobin subunits (4). Clinical treatments that are designed to alter the expression of specific hemoglobin subunits can be used to treat hemoglobinopathies (5). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 12 |
| 参考文献 | 1 . Hardison, R. (1998) J Exp Biol 201, 1099-117. 2 . Sankaran, V.G. et al. (2010) Br J Haematol 149, 181-94. 3 . Bank, A. (2006) Blood 107, 435-43. 4 . Thein, S.L. (2013) Cold Spring Harb Perspect Med 3, a011700. 5 . Fucharoen, S. et al. (1996) Blood 87, 887-92. |
Western blot analysis of purified human hemoglobin (lanes 2 and 3), and extracts from human whole blood (lane 4) and human liver (lane 5), using Hemoglobin β (D4W4I) Rabbit mAb. |
