| 货号 | 83348S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H |
| 来源宿主 | Rabbit IgG |
| 应用 | W IHC-P |
| 目标/特异性 | FGF19 (D1N3R) Rabbit mAb recognizes endogenous levels of total human FGF19 protein. This antibody does not cross-react with the mouse ortholog FGF15. |
| 使用方法 | WB(1:1000) IHC-P (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | FGF19 is a member of the large and diverse Fibroblast Growth Factor (FGF) family of peptide growth factors. FGF19 functions as a high affinity ligand for the FGF4 receptor, to which it binds in a heparin-dependent manner (1). Under normal physiological conditions, FGF19 is produced in the ileum when absorbed bile acids bind to the farnesoid X receptor (FXR), activating transcription of the FGF19 gene. FGF19 in turn functions in a negative feedback fashion to regulate bile acid synthesis (2). Consequently, disruptions in FGF19 signaling have been linked with clinical defects in bile acid synthesis, which may manifest as primary bile acid diarrhea or cholestasis (3,4). Research studies in oncology have shown that FGF19 can function in an endocrine, paracrine or autocrine fashion to promote tumorigenesis. In human studies, this has been demonstrated for breast cancer (5), gastric cancer (6) prostate cancer (7, 8), and hepatocellular carcinoma (9). Similar tumorigenic effects have been described for the mouse ortholog (Fgf15), notably in genetic models of hepatocellular carcinoma (10, 11) |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 22 |
| 参考文献 | 1 . Xie, M.H. et al. (1999) Cytokine 11, 729-35. 2 . Miyata, M. et al. (2012) J Steroid Biochem Mol Biol 132, 41-7. 3 . Walters, J.R. et al. (2009) Clin Gastroenterol Hepatol 7, 1189-94. 4 . Schaap, F.G. et al. (2009) Hepatology 49, 1228-35. 5 . Tiong, K.H. et al. (2016) Oncotarget 7, 57633-50. 6 . Wang, S. et al. (2016) Oncol Res 23, 197-203. 7 . Nagamatsu, H. et al. (2015) Prostate 75, 1092-101. 8 . Feng, S. et al. (2013) Cancer Res 73, 2551-62. 9 . Sawey, E.T. et al. (2011) Cancer Cell 19, 347-58. 10 . Desnoyers, L.R. et al. (2008) Oncogene 27, 85-97. 11 . Uriarte, I. et al. (2015) Int J Cancer 136, 2469-75. |
Immunohistochemical analysis of paraffin-embedded Colo 201 (left) and 293 (right) cell pellets using FGF19 (D1N3R) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human cholecystitis of the gall bladder using FGF19 (D1N3R) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human mucinous adenocarcinoma of the colon using FGF19 (D1N3R) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using FGF19 (D1N3R) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb (lower). |
