| 货号 | 79233S |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | H Mk |
| 来源宿主 | Mouse IgG1 |
| 应用 | W IP IF-IC F |
| 目标/特异性 | HIF-1α (D5F3M) Mouse mAb recognizes endogenous levels of total HIF-1α protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:1600) IF-IC (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism, and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VHL) E3 ligase complex; ubiquitination and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 120 |
| 参考文献 | 1 . Sharp, F.R. and Bernaudin, M. (2004) Nat Rev Neurosci 5, 437-48. 2 . Wang, G.L. et al. (1995) Proc Natl Acad Sci U S A 92, 5510-4. 3 . Jaakkola, P. et al. (2001) Science 292, 468-72. 4 . Maxwell, P.H. et al. (1999) Nature 399, 271-5. 5 . Fukuda, R. et al. (2002) J Biol Chem 277, 38205-11. 6 . Jiang, B.H. et al. (2001) Cell Growth Differ 12, 363-9. 7 . Laughner, E. et al. (2001) Mol Cell Biol 21, 3995-4004. 8 . Walisser, J.A. et al. (2004) Proc Natl Acad Sci U S A 101, 16677-82. 9 . Salomon-Nguyen, F. et al. (2000) Proc Natl Acad Sci U S A 97, 6757-62. 10 . Gunton, J.E. et al. (2005) Cell 122, 337-49. |
Confocal immunofluorescent analysis of Hep G2 cells, untreated (left) or treated with cobalt chloride (500 μM, 24 h; right), using HIF-1α (D5F3M) Mouse mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). | |
Flow cytometric analysis of U-2 OS cells, untreated (blue) or treated with DMOG (1mM, 6 h; green) using HIF-1α (D5F3M) Mouse mAb. Anti-mouse IgG (H+L), F(ab)2 fragment (Alexa Fluor® 488 conjugate) #4408 was used as a secondary antibody. | |
Western blot analysis of extracts from Hep G2 cells untreated or treated with cobalt chloride (100 µM, 4 h; +) and U-2 OS cells untreated or treated with DMOG (1 mM, 6 h; +) using HIF-1α Mouse (D5F3M) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). |
