| 货号 | 77510S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | IRG1 (D6H2Y) Rabbit mAb recognizes endogenous levels of total IRG1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | IRG1 (Immune-responsive gene 1) is one of the most up-regulated genes in macrophages under proinflammatory conditions (1). It is also highly expressed in the pregnant uterus during implantation (2,3). IRG1 is a cis-aconitate decarboxylase that produces itaconic acid by decarboxylating cis-aconic acid, an intermediate of the tricarboxylic acid cycle (4). Itaconic acid is an endogenous inhibitor of succinate dehydrogenase, linking macrophage metabolic rewiring and regulation of inflammation (5,6). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 53 |
| 参考文献 | 1 . Lee, C.G. et al. (1995) Immunogenetics 41, 263-70. 2 . Chen, B. et al. (2003) Mol Endocrinol 17, 2340-54. 3 . Cheon, Y.P. et al. (2003) Endocrinology 144, 5623-30. 4 . Michelucci, A. et al. (2013) Proc Natl Acad Sci U S A 110, 7820-5. 5 . Lampropoulou, V. et al. (2016) Cell Metab 24, 158-66. 6 . Cordes, T. et al. (2016) J Biol Chem 291, 14274-84. |
Western blot analysis of lysates from THP-1 untreated (-) or treated with TPA (#4174, 50ng/ml) for 24 hours then LPS (5ug/ml) for 6 hours in fresh medium (+), using IRG1 (D6H2Y) Rabbit mAb (upper) or GAPDH (14C10) Rabbit mAb #2118 (lower). |
