IκB-ζ (D4I7C) Rabbit mAb (ChIP Preferred)【科瑞奥博】

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IκB-ζ (D4I7C) Rabbit mAb (ChIP Preferred)

货号： 76041S 基本售价： 3360.0 元规格： -

产品信息

概述
货号	76041S
同种亚型	Rabbit IgG
反应种属	M
来源宿主	Rabbit IgG
应用	W IP ChIP
目标/特异性	IκB-ζ (D4I7C) Rabbit mAb (ChIP Preferred) recognizes endogenous levels of total mouse IκB-ζ protein. This antibody has weak reactivity for rat and human. Product #93726 is preferred for western blot.
使用方法	WB(1:1000) IP (1:50) ChIP (1:50)

性能
供应商	CST
灵敏度	Endogenous
背景	The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
运输条件	0.75
存放说明	-20C
计算分子量	75, 85
参考文献	1 . Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6. 2 . Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70. 3 . Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8. 4 . Brown, K. et al. (1995) Science 267, 1485-8. 5 . Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18. 6 . Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. 7 . Chen, Z.J. et al. (1996) Cell 84, 853-62. 8 . Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63. 9 . Yamazaki, S. et al. (2001) J Biol Chem 276, 27657-62. 10 . Kitamura, H. et al. (2000) FEBS Lett 485, 53-6. 11 . Haruta, H. et al. (2001) J Biol Chem 276, 12485-8. 12 . Matsuo, S. et al. (2007) Biochem J 405, 605-15. 13 . Totzke, G. et al. (2006) J Biol Chem 281, 12645-54. 14 . Motoyama, M. et al. (2005) J Biol Chem 280, 7444-51. 15 . Yamamoto, M. et al. (2004) Nature 430, 218-22.

参考图片

Western blot analysis of extracts from J774A.1 and Raw 264.7 cell lines, untreated (-) or treated with LPS (1 μg/ml, 4 hr; +) using IκB-ζ (D4I7C) Rabbit mAb (ChIP Preferred) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length mouse IκB-ζ (mIκB-ζ; +) using IκB-ζ (D4I7C) Rabbit mAb (ChIP Preferred).

Immunoprecipitation of IκB-ζ from Raw 264.7 cells treated with LPS (1 μg/ml; 4 hr). Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900, and lane 3 is IκB-ζ (D4I7C) Rabbit mAb (ChIP Preferred). Western blot was performed using IκB-ζ (D4I7C) Rabbit mAb (ChIP Preferred). Blots were developed using a confirmation specific secondary antibody to avoid reactivity with IgG.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10⁶ J774A.1 cells treated with LPS #14011 (1μg/mL, 4hours) and either 10 µl of IκB-ζ (D4I7C) Rabbit mAb (Mouse Specific) or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP^® Mouse CXCL2 Promoter Primers #39609, mouse LCN2 promoter primers, and SimpleChIP^® Mouse MYT-1 Promoter Primers #8985. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.