| 货号 | 70180S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H Mk |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | LARP1 (D8J4F) Rabbit mAb recognizes endogenous levels of total LARP1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | La-related protein 1 (LARP1) is a ubiquitously expressed RNA binding protein that promotes both global and specific mRNA translation in cells (1). LARP1 belongs to the La-related protein family and contains two RNA binding domains, a La motif (LAM), and a neighboring RNA recognition motif-like (RRM-L) domain (1). Research studies indicate that LARP1 acts downstream of mTORC1 to facilitate cell proliferation and growth by promoting global mRNA translation and translation of mRNAs containing a 5Terminal Oligo-Pyrimidine (5TOP) motif, which code for translational machinery components (2,3). At the molecular level, LARP1 associates with 5TOP mRNAs and multiple translation machinery components to positively regulate translation (2,4). Additional studies show that LARP1 expression is upregulated in hepatocellular carcinoma (HCC) patients and that high LARP1 expression in HCC negatively correlates with survival rate (5). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 150 |
| 参考文献 | 1 . Bayfield, M.A. et al. (2010) Biochim Biophys Acta 1799, 365-78. 2 . Tcherkezian, J. et al. (2014) Genes Dev 28, 357-71. 3 . Burrows, C. et al. (2010) Nucleic Acids Res 38, 5542-53. 4 . Aoki, K. et al. (2013) FEBS Lett 587, 2173-8. 5 . Xie, C. et al. (2013) J Transl Med 11, 272. |
Western blot analysis of extracts from various cell lines using LARP1 (D8J4F) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with siRNA targeting human LARP1 (+), using LARP1 (D8J4F) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). | |
Immunoprecipitation of LARP1 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is immunoprecipitation with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is immunoprecipitation with LARP1 (D8J4F) Rabbit mAb. Western blot analysis was performed using LARP1 (D8J4F) Rabbit mAb. |
