| 货号 | 65344S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H M R Mk |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | UBR5 (D6O8Z) Rabbit mAb recognizes endogenous levels of total UBR5 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Human E3 identified by differential display (UBR5/EDD) is a HECT domain-containing ubiquitin E3 ligase of the N-end rule pathway that promotes the ubiquitination and proteasomal degradation of proteins harboring N-degrons (1-3). UBR5 represents an ortholog of HYD, the Drosophilahyperplastic discs tumor suppressor gene product but has been found to be overexpressed in breast and ovarian cancers, suggesting a possible role in promoting tumor development (4,5). Research studies have demonstrated that UBR5 is functional within the nucleus as it participates in DNA damage signaling by controlling the activities of Chk2, TopBP1, and RNF168 (6-9). Recently, UBR5 was shown to play a novel role in immune cell function by regulating RORγt stability and IL-17 production by Th17 cells (10). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 300 |
| 参考文献 | 1 . Sriram, S.M. et al. (2011) Nat Rev Mol Cell Biol 12, 735-47. 2 . Callaghan, M.J. et al. (1998) Oncogene 17, 3479-91. 3 . Tasaki, T. et al. (2005) Mol Cell Biol 25, 7120-36. 4 . Clancy, J.L. et al. (2003) Oncogene 22, 5070-81. 5 . OBrien, P.M. et al. (2008) Br J Cancer 98, 1085-93. 6 . Munoz, M.A. et al. (2007) Cell Cycle 6, 3070-7. 7 . Henderson, M.J. et al. (2006) J Biol Chem 281, 39990-40000. 8 . Honda, Y. et al. (2002) J Biol Chem 277, 3599-605. 9 . Gudjonsson, T. et al. (2012) Cell 150, 697-709. 10 . Rutz, S. et al. (2015) Nature 518, 417-21. |
Western blot analysis of extracts from various cell lines using UBR5 (D6O8Z) Rabbit mAb. | |
Immunoprecipitation of UBR5 from MCF7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is UBR5 (D6O8Z) Rabbit mAb. Western blot analysis was performed using UBR5 (D6O8Z) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with siRNA targeting human UBR5 (+), using UBR5 (D6O8Z) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). |
