| 货号 | 60758S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H M R |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | NAF-1/CISD2 (D4X4E) Rabbit mAb recognizes endogenous levels of total NAF-1/CISD2 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Nutrient-deprivation autophagy factor-1 (NAF-1) (also known as CISD2, CDGSH iron-sulfur domain-containing protein 2) is a member of NEET family of 2Fe-2S proteins, characterized by a unique CDGSH sequence at their Fe-S-cluster-binding domain (1). NAF-1/CISD2 is a multifunctional protein. In addition to its role in iron and ROS homeostasis, it has been shown to play a role in autophagy, neurodegenerative diseases, and aging (2-7). Enhanced expression of NAF-1/CISD2 is associated with many types of cancer. Silencing of NAF-1/CISD2 expression in cancer cells significantly inhibited proliferation and tumorigenicity; while overexpression of NAF-1/CISD2 significantly enhanced proliferation (2, 8, 9). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 15 |
| 参考文献 | 1 . Tamir, S. et al. (2015) Biochim Biophys Acta 1853, 1294-315. 2 . Chang, N.C. et al. (2012) Autophagy 8, 856-7. 3 . Tamir, S. et al. (2015) Biochim Biophys Acta 1853, 1294-315. 4 . Lipper, C.H. et al. (2015) PLoS One 10, e0139699. 5 . Du, X. et al. (2015) Cell Biol Int 39, 816-23. 6 . Chen, Y.F. et al. (2009) Genes Dev 23, 1183-94. 7 . Chen, Y.F. et al. (2010) Ann N Y Acad Sci 1201, 58-64. 8 . Yang, L. et al. (2016) Oncotarget 7, 22720-32. 9 . Holt, S.H. et al. (2016) J Cell Sci 129, 155-65. |
Western blot analysis of extracts from various cell lines using NAF-1/CISD2 (D4X4E) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). |
