| 货号 | 25656S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IHC-P IF-IC |
| 目标/特异性 | Stat5 (D3N2B) recognizes endogenous levels of total Stat5 protein.This antibody preferentially detects Stat5a, but may have weak reactivity for Stat5b. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:200) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 90 |
| 参考文献 | 1 . Gouilleux, F. et al. (1994) EMBO J 13, 4361-9. 2 . Wakao, H. et al. (1994) EMBO J 13, 2182-91. 3 . Okutani, Y. et al. (2001) Oncogene 20, 6643-50. 4 . Demoulin, J.B. et al. (1999) J Biol Chem 274, 25855-61. 5 . Dentelli, P. et al. (1999) J Immunol 163, 2151-9. 6 . Meinke, A. et al. (1996) Mol Cell Biol 16, 6937-44. |
Confocal immunofluorescent analysis of Malme-3M (left) and PC-3 (right) cells using Stat5 (D3N2B) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin (red). | |
Immunohistochemical analysis of paraffin-embedded K-562 (left) and PC-3 (right) cell pellets using Stat5 (D3N2B) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Stat5 (D3N2B) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using Stat5 (D3N2B) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of PC-3 cells, mock transfected (-) or transfected with constructs expressing full-length human Stat5a (+) or Stat5b (+) using Stat5 (D3N2B) Rabbit mAb. | |
Immunoprecipitation of Stat5a from K-562 cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Stat5 (D3N2B) Rabbit mAb. Western blot was performed using Stat5 (D3N2B) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human infiltrating papillary carcinoma of the breast using Stat5 (D3N2B) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Stat5 (D3N2B) Rabbit mAb. |
