| 货号 | 20112S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | M R |
| 来源宿主 | Rabbit IgG |
| 应用 | IF-F IF-IC |
| 目标/特异性 | AE1/SLC4A1 (D3X1R) Rabbit mAb (IF Formulated; Rodent Specific) recognizes endogenous levels of total AE1 protein. |
| 使用方法 | IF-F(1:200) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Anion exchange protein 1 (AE1), also named solute carrier family 4 member 1 (SLC4A1), is an anion transporter that mediates chloride-bicarbonate exchange in the kidney and regulates normal acidification of the urine (1,2). A different isoform of AE1 is a major integral membrane structure protein of erythrocytes, where it plays a critical role in the removal of carbon dioxide from tissues (3). In addition, AE1 is required for normal flexibility and stability of the erythrocyte membrane. Mutations in SLC4A1 can lead to hereditary spherocytosis, ovalocytosis, and distal renal tubular-acidosis (4-7). Other mutations that do not cause disease became novel blood group antigens, which are part of the Diego blood group system (8). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 参考文献 | 1 . Kopito, R.R. and Lodish, H.F. Nature 316, 234-8. 2 . Kollert-Jöns, A. et al. (1993) Am J Physiol 265, F813-21. 3 . Swenson, E.R. et al. (1993) J Appl Physiol (1985) 74, 838-48. 4 . Jarolim, P. et al. (1991) Proc Natl Acad Sci U S A 88, 11022-6. 5 . Maillet, P. et al. (1995) Br J Haematol 91, 804-10. 6 . Quilty, J.A. et al. (2002) Am J Physiol Renal Physiol 282, F810-20. 7 . Escobar, L. et al. (2013) Nefrologia 33, 289-96. 8 . Figueroa, D. (2013) Immunohematology 29, 73-81. |
Confocal immunofluorescent analysis of mouse kidney using AE1/SLC4A1 (D3X1R) Rabbit mAb (IF Formulated; Rodent Specific) (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Note AE1 expression in intercalated cells of the collecting duct (left) and erythrocytes (right). | |
Confocal immunofluorescent analysis of mouse whole blood using AE1/SLC4A1 (D3X1R) Rabbit mAb (IF Formulated; Rodent Specific) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Note: AE1 expression in enucleated erythrocytes only. |
