| 货号 | 20087S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H R Mk |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | OTUD5 (D8Y2U) Rabbit mAb recognizes endogenous levels of total OTUD5 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes and deubiquitinating enzymes, respectively (1,2). Deubiquitinating enzymes (DUBs) are categorized into five subfamilies based on catalytic domain structure: USP, OTU, MJD, UCH, and JAMM/MPN. OTUD5, also known as DUBA, belongs to the OTU family of DUBs. Phosphorylation of OTUD5 at Ser177 is required to activate its DUB activity (3). Research studies have shown that OTUD5 plays a pivotal role in negatively regulating innate immune responses though its ability to bind and deubiquitinate K63-linked TRAF3, thereby suppressing production of IFN-I by macrophages (4). There is also evidence that OTUD5 participates in the adaptive immune response by negatively regulating the production of pro-inflammatory IL-17 by Th17 cells (5). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 75 |
| 参考文献 | 1 . Nijman, S.M. et al. (2005) Cell 123, 773-86. 2 . Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613. 3 . Huang, O.W. et al. (2012) Nat Struct Mol Biol 19, 171-5. 4 . Kayagaki, N. et al. (2007) Science 318, 1628-32. 5 . Rutz, S. et al. (2015) Nature 518, 417-21. |
Western blot analysis of extracts from various cell lines using OTUD5 (D8Y2U) Rabbit mAb. | |
Immunoprecipitation of OTUD5 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is OTUD5 (D8Y2U) Rabbit mAb. Western blot analysis was performed using OTUD5 (D8Y2U) Rabbit mAb. Anti-Rabbit HRP-conjugated light chain specific secondary antibody was used for detection. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with siRNA targeting human OTUD5 (+), using OTUD5 (D8Y2U) Rabbit mAb (upper) and GAPDH (D16H11) XP®Rabbit mAb #5174 (lower). |
