| 货号 | 57156S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H M R Mk |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IF-IC ChIP |
| 目标/特异性 | HDAC2 (D6S5P) recognizes endogenous levels of total HDAC2 protein. This antibody does not cross-react with HDAC1. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:1000) ChIP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 60 |
| 参考文献 | 1 . Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703. 2 . Gregory, P.D. et al. (2001) Exp Cell Res 265, 195-202. 3 . Liu, Y. et al. (2000) Mol Cell Biol 20, 5540-53. 4 . Cress, W.D. and Seto, E. (2000) J Cell Physiol 184, 1-16. 5 . Gray, S.G. and Ekström, T.J. (2001) Exp Cell Res 262, 75-83. 6 . Thiagalingam, S. et al. (2003) Ann. N.Y. Acad. Sci. 983, 84-100. 7 . Vigushin, D.M. and Coombes, R.C. (2004) Curr Cancer Drug Targets 4, 205-18. |
Western blot analysis of extracts from various cell lines using HDAC2 (D6S5P) Rabbit mAb. | |
Immunoprecipitation of HDAC2 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is HDAC2 (D6S5P) Rabbit mAb. Western blot analysis was performed using HDAC2 (D6S5P) Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa cells using HDAC2 (D6S5P) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 K562 cells and either 10 µl of HDAC2 (D6S5P) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human RPL30 Exon 3 Primers #7014, human BCR exon 1 primers, and SimpleChIP® Human Nanog Promoter Primers #95064. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
