| 货号 | 56044S |
| 同种亚型 | Mouse IgG2b |
| 反应种属 | H M |
| 来源宿主 | Mouse IgG2b |
| 应用 | W IP F |
| 目标/特异性 | BTK (D6T2C) Mouse mAb recognizes endogenous levels of total Btk protein. The antibody is predicted to recognize two known Btk isoforms (Btk-A and Btk-C), which are derived from the same gene, but regulated by alternative promoter usage. |
| 使用方法 | WB(1:1000) IP (1:200) F (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Brutons tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. Btk plays an important role in B cell development (1,2). Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate (3-5). The membrane-localized Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinases, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation (6,7). The activation of Btk is negatively regulated by PKCβ through phosphorylation of Btk at Ser180, which results in reduced membrane recruitment, transphosphorylation, and subsequent activation (8). The PKC inhibitory signal is likely to be a key determinant of the B cell receptor signaling threshold to maintain optimal Btk activity (8). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 78 |
| 参考文献 | 1 . Khan, W.N. (2001) Immunol Res 23, 147-56. 2 . Lewis, C.M. et al. (2001) Curr Opin Immunol 13, 317-25. 3 . Salim, K. et al. (1996) EMBO J 15, 6241-50. 4 . Rameh, L.E. et al. (1997) J Biol Chem 272, 22059-66. 5 . Várnai, P. et al. (1999) J Biol Chem 274, 10983-9. 6 . Rawlings, D.J. et al. (1996) Science 271, 822-5. 7 . Park, H. et al. (1996) Immunity 4, 515-25. 8 . Kang, S.W. et al. (2001) EMBO J 20, 5692-702. |
Flow cytometric analysis of Jurkat cells (blue) and Ramos cells (green) using BTK (D6T2C) Mouse mAb. Anti-mouse IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody. | |
Western blot analysis of extracts from various cell lines using Btk (D6T2C) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Extracts from T cells (Jurkat and MOLT4) are negative for Btk expression, demonstrating specificity of the antibody. | |
Immunoprecipitation of Btk from Ramos cell extracts. Lane 1 is 10% input, lane 2 is Mouse (G3A1) mAb IgG1 Isotype Control #5415, and lane 3 is Btk (D6T2C) Mouse mAb. Western blot analysis was performed with Btk (D6T2C) Rabbit mAb #8547. |
