| 货号 | 55935S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H Mk |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | SH3BP4 (D3D9I) Rabbit mAb recognizes endogenous levels of total SH3BP4 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by enviromental factors such as energy levels, growth factors, and amino acids (3, 4). The GTPases RagA, RagB, RagC, and RagD mediate amino acid signaling to activate mTORC1 (1, 2). SH3BP4 (SH3 domain-binding protein 4) binds to the inactive Rag GTPase complex during amino acid starvation and prevents the association of Rag GTPase complex with mTORC1 resulting in the suppression of mTORC1 activation and cell growth (5). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 110 |
| 参考文献 | 1 . Sancak, Y. et al. (2008) Science 320, 1496-501. 2 . Kim, E. et al. (2008) Nat Cell Biol 10, 935-45. 3 . Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45. 4 . Wullschleger, S. et al. (2006) Cell 124, 471-84. 5 . Kim, Y.M. et al. (2012) Mol Cell 46, 833-46. |
Western blot analysis of extracts from various cell lines using SH3BP4 (D3D9I) Rabbit mAb. | |
Immunoprecipitation of SH3BP4 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is SH3BP4 (D3D9I) Rabbit mAb. Western blot analysis was performed using SH3BP4 (D3D9I) Rabbit mAb. |
