| 货号 | 54037S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H Mk |
| 来源宿主 | Rabbit IgG |
| 应用 | W |
| 目标/特异性 | C17orf59 (D2B5X) Rabbit mAb recognizes endogenous levels of total C17orf59 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | mTORC1 kinase complex is a critical component to regulate cell growth in response to energy levels, growth factors, and amino acids (1-4). The GTPases RagA, RagB, RagC and RagD have been shown to interact with raptor in mTORC1 (3,4). A Ragulator complex, consisting of LAMTOR1/C11orf59, LAMTOR2/ROBLD3, LAMTOR3/MAPKSP1, LAMTOR4/C7orf59 and LAMTOR5/HBXIP, associates with the Rag GTPases and recruit them to the surface of lysosomes (5, 6). These interactions lead to mTORC1 activation in response to amino acid signals (3-6). In addition, C17orf59 was shown to interact with Ragulator and disrupt the interaction between Ragulator and Rag GTPases (7). This disruption prevents mTOR from localizing to the lysosomal membrane and therefore inhibits mTORC1 activation (7). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 42 |
| 参考文献 | 1 . Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45. 2 . Wullschleger, S. et al. (2006) Cell 124, 471-84. 3 . Sancak, Y. et al. (2008) Science 320, 1496-501. 4 . Kim, E. et al. (2008) Nat Cell Biol 10, 935-45. 5 . Sancak, Y. et al. (2010) Cell 141, 290-303. 6 . Bar-Peled, L. et al. (2012) Cell 150, 1196-208. 7 . Schweitzer, L.D. et al. (2015) Cell Rep 12, 1445-55. |
Western blot analysis of extracts from various cell lines using C17orf59 (D2B5X) Rabbit mAb. |
