| 货号 | 50353S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H M |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IHC-P |
| 目标/特异性 | HSP27 (E1J4D) Rabbit mAb (IHC Preferred) recognizes endogenous levels of total HSP27 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 27 |
| 参考文献 | 1 . Arrigo, A.P. and Landry, J. (1994) Cold Spring Harbor Laboratory Press, NY, 335-373. 2 . Landry, J. et al. (1992) J. Biol. Chem. 267, 794-803. 3 . Rouse, J. et al. (1994) Cell 78, 1027-1037. 4 . Rogalla, T. et al. (1999) J. Biol. Chem. 274, 18947-18956. 5 . Lavoie, J. et al. (1993) J. Biol. Chem. 268, 24210-24214. 6 . Rousseau, S. et al. (1997) Oncogene 15, 2169-2177. |
Immunohistochemical analysis of paraffin-embedded HeLa (left) and HT-1080 (right) cell pellets using HSP27 (E1J4D) Rabbit mAb (IHC Preferred). | |
Immunohistochemical analysis of paraffin-embedded mouse forestomach using HSP27 (E1J4D) Rabbit mAb (IHC Preferred). | |
Western blot analysis of extracts from various cell lines using HSP27 (E1J4D) Rabbit mAb (IHC Preferred) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of HSP27 from ACHN cell extracts. Lane 1 is 10% input, lane 2 is immunoprecipitated with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is HSP27 (E1J4D) Rabbit mAb (IHC Preferred). Western blot was performed using HSP27 (E1J4D) Rabbit mAb (IHC Preferred). A conformation specific secondary antibody was used to avoid cross reactivity with IgG. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HSP27 (E1J4D) Rabbit mAb (IHC Preferred). | |
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the lung using HSP27 (E1J4D) Rabbit mAb (IHC Preferred). | |
Immunohistochemical analysis of paraffin-embedded human non-Hodgkins lymphoma using HSP27 (E1J4D) Rabbit mAb (IHC Preferred). |
