| 货号 | 46743S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H M R Mk |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP ChIP |
| 目标/特异性 | NRF1 (D9K6P) Rabbit mAb recognizes endogenous levels of total NRF1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) ChIP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Nuclear respiratory factor 1 (NRF1) was identified as a transcription activator for the gene encoding cytochrome c (1). It was later found to play a role in the nuclear control of mitochondrial function (1). PGC-1 induces the expression of NRF1 and NRF2 (2). NRF1, along with the coactivator PGC-1, stimulates the promoter of mitochondrial transcription factor A, which regulates mitochondrial biogenensis and function (2). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 68 |
| 参考文献 | 1 . Virbasius, C.A. et al. (1993) Genes Dev 7, 2431-45. 2 . Wu, Z. et al. (1999) Cell 98, 115-24. |
Western blot analysis of extracts from various cell lines using NRF1 (D9K6P) Rabbit mAb. | |
Immunoprecipitation of NRF1 from HepG2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is NRF1 (D9K6P) Rabbit mAb. Western blot analysis was performed using NRF1 (D9K6P) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 293 cells and either 5 µl of NRF1 (D9K6P) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human MEF2A Exon 1 Primers #26727, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
