| 货号 | 46477S |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | H |
| 来源宿主 | Mouse IgG1 |
| 应用 | W IHC-P |
| 目标/特异性 | HSP70 (D1M6J) Mouse mAb recognizes endogenous levels of total HSP70 protein. This antibody does not cross-react with Heat shock-related 70 kDa protein 2 (HSPA2) or Heat shock cognate 71 kDa protein (HSC70/HSPA8). |
| 使用方法 | WB(1:1000) IHC-P (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | HSP70 and HSP90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress (1). Both HSP70 and HSP90 are able to interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of their substrates in an ATP- and co-chaperone-dependent manner (1). HSP70 has a broad range of substrates including newly synthesized and denatured proteins, while HSP90 tends to have a more limited subset of substrates, most of which are signaling molecules. HSP70 and HSP90 often function collaboratively in a multi-chaperone system, which requires a minimal set of co-chaperones: HSP40, Hop, and p23 (2,3). The co-chaperones either regulate the intrinsic ATPase activity of the chaperones or recruit chaperones to specific substrates or subcellular compartments (1,4). When the ubiquitin ligase CHIP associates with the HSP70/HSP90 complex as a cofactor, the unfolded substrates are subjected to degradation by the proteasome (4). The biological functions of HSP70/HSP90 extend beyond their chaperone activity. They are essential for the maturation and inactivation of nuclear hormones and other signaling molecules (1,3). They also play a role in vesicle formation and protein trafficking (2). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 70 |
| 参考文献 | 1 . Nollen, E.A. and Morimoto, R.I. (2002) J. Cell Sci. 115, 2809-2816. 2 . Young, J.C. et al. (2003) Trends Biochem. Sci. 28, 541-547. 3 . Pratt, W.B. and Toft, D.O. (2003) Exp. Biol. Med. 228, 111-133. 4 . Hohfeld, J. et al. (2001) EMBO Rep. 2, 885-890. |
Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, untreated (left) or heat shock-treated (right), using HSP70 (D1M6J) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human lymphoma using HSP70 (D1M6J) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using HSP70 (D1M6J) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using HSP70 (D1M6J) Mouse mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected or transfected with human HSPA1A-Myc/DDK, HSPA2-MycDDK or HSPA8-Myc/DDK, using HSP70 (D1M6J) Mouse mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower). | |
Western blot analysis of extracts from various cell lines using HSP70 (D1M6J) Mouse mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower). |
