| 货号 | 46307S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | IL-2Rβ (D4X3H) Rabbit mAb recognizes endogenous levels of total IL-2Rβ protein. This antibody cross-reacts with proteins of unknown identity at 38-42 kDa. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ (1,2). γc is used by other cytokines including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2). IL-2Rβ, also known as CD122, is a type I transmembrane protein that is expressed by natural killer cells and certain subsets of T cells in the periphery (6). IL-2Rβ surface expression is upregulated following antigen-induced activation. A heteromeric complex composed of IL-2Rβ and γc forms the intermediate affinity IL-2 receptor whereas a complex comprised of all three chains forms the high affinity receptor. Signaling through IL-2Rβ is mediated by Jak1 (7), which phosphorylates numerous tyrosine residues in the cytoplasmic tail to promote the recruitment of the adaptor Shc to IL-2Rβ and enhance IL-2 receptor signaling (8, 9). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 79 |
| 参考文献 | 1 . Ma, A. et al. (2006) Annu Rev Immunol 24, 657-79. 2 . Gaffen, S.L. and Liu, K.D. (2004) Cytokine 28, 109-23. 3 . Fehérvari, Z. et al. (2006) Trends Immunol 27, 109-11. 4 . Antony, P.A. et al. (2006) J Immunol 176, 5255-66. 5 . Jaleco, S. et al. (2003) J Immunol 171, 61-8. 6 . Shevach, E.M. (2012) Trends Immunol 33, 626-32. 7 . Nelson, B.H. and Willerford, D.M. (1998) Adv Immunol 70, 1-81. 8 . Gaffen, S.L. (2001) Cytokine 14, 63-77. 9 . Yu, A. et al. (2009) Immunity 30, 204-17. |
Western blot analysis of extracts from various cell lines using IL-2Rβ (D4X3H) Rabbit mAb (upper), and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of IL-2Rβ protein from HDLM-2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP®Isotype Control #3900, and lane 3 is IL-2Rβ (D4X3H) Rabbit mAb. Western blot analysis was performed using IL-2Rβ (D4X3H) Rabbit mAb. |
