| 货号 | 45844S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H M R |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | CRYAB (D6S9E) Rabbit mAb recognizes endogenous levels of total CRYAB protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CRYAB (αB-Crystallin) is a member of the small heat shock protein (sHSP also known as HSP20) family (1). This protein was initially found to be overexpressed in the eye lens, and later also detected at high levels in heart and skeletal muscle tissues (2,3). CRYAB functions mainly as a molecular chaperone, responding to stress by binding unfolded target proteins to prevent aggregation (4,5). Research studies have shown that elevated expression of CRYAB in neurological disease and stroke patients protects tissue and cells from damage under extreme stress, leading to the investigation of CRYAB as a potential therapeutic target (6-9). Researchers also found that expression of the missense mutation of CRYAB (R120G) in the mouse model causes cardiomyopathy due to abnormal desmin aggregation (10). At the molecular level, CRYAB is involved in multiple biological processes, such as inhibiting apoptosis by binding and inhibiting caspase and proapoptotic Bax and Bcl-xS protein functions (11,12), promoting angiogenesis by binding and stabilizing VEGF for secretion (13), and regulating cytoskeletal organization through association with actin filament, intermediate filament, and cardiac titin (14-16). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 22 |
| 参考文献 | 1 . Mehlen, P. et al. (1996) EMBO J 15, 2695-706. 2 . Piatigorsky, J. (1989) FASEB J 3, 1933-40. 3 . Bennardini, F. et al. (1992) Circ Res 71, 288-94. 4 . Benjamin, I.J. and McMillan, D.R. (1998) Circ Res 83, 117-32. 5 . Horwitz, J. (1992) Proc Natl Acad Sci U S A 89, 10449-53. 6 . Ousman, S.S. et al. (2007) Nature 448, 474-9. 7 . Ray, P.S. et al. (2001) FASEB J 15, 393-402. 8 . Arac, A. et al. (2011) Proc Natl Acad Sci U S A 108, 13287-92. 9 . Sanbe, A. (2011) Biol Pharm Bull 34, 1653-8. 10 . Wang, X. et al. (2001) Circ Res 89, 84-91. 11 . Kamradt, M.C. et al. (2001) J Biol Chem 276, 16059-63. 12 . Mao, Y.W. et al. (2004) Cell Death Differ 11, 512-26. 13 . Kase, S. et al. (2010) Blood 115, 3398-406. 14 . Wang, K. and Spector, A. (1996) Eur J Biochem 242, 56-66. 15 . Nicholl, I.D. and Quinlan, R.A. (1994) EMBO J 13, 945-53. 16 . Zhu, Y. et al. (2009) J Biol Chem 284, 13914-23. |
Western blot analysis of extracts from various cell lines using CRYAB (D6S9E) Rabbit mAb. | |
Immunoprecipitation of CRYAB protein from ACHN cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CRYAB (D6S9E) Rabbit mAb. Western blot analysis was performed using CRYAB (D6S9E) Rabbit mAb. |
