| 货号 | 42299S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IHC-P IF-IC |
| 目标/特异性 | NaPi2b/SLC34A2 (D6W2G) Rabbit mAb |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The sodium-dependent phosphate transport protein 2B (NaPi-2b, SLC34A2) is a sodium dependent inorganic phosphate (Pi) transporter that regulates phosphate homeostasis in various organs, including the small intestine, lung, liver, and testis (1). In the small intestine, NaPi-2b localizes to the intestinal brush border membrane to mediate Pi reabsorption (2). In the lung, NaPi-2b is expressed in the apical membrane of type II alveolar cells and is involved in the synthesis of surfactant (3). Mutations in the corresponding SLC34A2 gene causes pulmonary alveolar microlithiasis, a rare autosomal recessive disorder characterized by the deposition of calcium phosphate microliths throughout the lungs (4). Research studies show aberrant expression of NaPi-2b in various type of cancer, including ovarian, breast, and lung cancer (5). Chromosomal rearrangements involving SLC34A2-ROS1 are seen in gastric carcinoma and non-small cell lung cancer and result in the formation of a SLC34A2-ROS1 chimeric protein that retains a constitutive kinase activity (6,7). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 73, 90-130 |
| 参考文献 | 1 . Xu, H. et al. (1999) Genomics 62, 281-4. 2 . Hilfiker, H. et al. (1998) Proc Natl Acad Sci U S A 95, 14564-9. 3 . Traebert, M. et al. (1999) Am J Physiol 277, L868-73. 4 . Yin, X. et al. (2013) Respir Med 107, 217-22. 5 . Kiyamova, R. et al. (2011) Exp Oncol 33, 157-61. 6 . Davies, K.D. et al. (2012) Clin Cancer Res 18, 4570-9. 7 . Lee, J. et al. (2013) Cancer 119, 1627-35. |
Confocal immunofluorescent analysis of IGROV-1 (left) and Hep G2 (right) cells using NaPi2b/SLC34A2 (D6W2G) Rabbit mAb (green). Actin filaments were labeled with DyLightTM 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from IGROV1, ACHN and HepG2 using NaPi2b/SLC34A2 (D6W2G) Rabbit mAb and beta-Actin (13E5) Rabbit mAb #4970. | |
Immunohistochemical analysis of paraffin-embedded HCC78 (left) and Hep G2 (right) cell pellets using NaPi2b/SLC34A2 (D6W2G)Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using NaPi2b/SLC34A2 (D6W2G) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using NaPi2b/SLC34A2 (D6W2G) Rabbit mAb. |
