| 货号 | 35672S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H M R |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IF-IC |
| 目标/特异性 | CRMP-2 (D8L6V) Rabbit mAb recognizes endogenous levels of total CRMP-2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Collapsin Response Mediator Protein-2 (CRMP-2) is expressed at high levels in the developing nervous system and plays a critical role in axonal outgrowth by specifying axon/dendrite fate and establishing neuronal polarity (1,2). CRMP-2 enhances axon elongation and branching by binding to tubulin heterodimers to promote microtubule assembly (3). GSK-3β inactivates CRMP-2 by phosphorylating it at Thr514. CRMP-2 is primed following phosphorylation at Ser522 by CDK5 and at Thr518 by GSK-3β (2). Phosphorylation of CRMP-2, which decreases tubulin binding ability, can be inhibited by NT-3 and BDNF through the PI3 kinase/Akt pathway (2). CRMP-2 also mediates semaphorin-induced growth cone collapse (4). Hyperphosphorylation of CRMP-2 is found in Alzheimer disease plaques with concurrent elevated GSK-3β activity in these patients (5). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 60-80 |
| 参考文献 | 1 . Gu, Y. and Ihara, Y. (2000) J Biol Chem 275, 17917-20. 2 . Yoshimura, T. et al. (2005) Cell 120, 137-49. 3 . Fukata, Y. et al. (2002) Nat Cell Biol 4, 583-91. 4 . Goshima, Y. et al. (1995) Nature 376, 509-14. 5 . Cole, A.R. et al. (2004) J Biol Chem 279, 50176-80. |
Confocal immunofluorescent analysis of SH-SY5Y (upper) and NCI-H522 (lower) cells using CRMP-2 (D8L6V) Rabbit mAb (green). Actin filaments have been labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines and tissues using CRMP-2 (D8L6V) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of CRMP-2 from SH-SY5Y extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CRMP-2 (D8L6V) Rabbit mAb. Western blot analysis was performed using CRMP-2 (D8L6V) Rabbit mAb. |
