| 货号 | 35362S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H B |
| 来源宿主 | Rabbit IgG |
| 应用 | W IHC-P |
| 目标/特异性 | eNOS (D8A6N) Rabbit mAb recognizes endogenous levels of total eNOS protein. This antibody does not cross-react with iNOS or nNOS proteins. |
| 使用方法 | WB(1:1000) IHC-P (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 140 |
| 参考文献 | 1 . Fulton, D. et al. (2001) J Pharmacol Exp Ther 299, 818-24. 2 . Shaul, P.W. (2002) Annu Rev Physiol 64, 749-74. 3 . Chen, Z.P. et al. (1999) FEBS Lett 443, 285-9. 4 . Dimmeler, S. et al. (1999) Nature 399, 601-5. 5 . Fulton, D. et al. (1999) Nature 399, 597-601. 6 . Harris, M.B. et al. (2001) J Biol Chem 276, 16587-91. 7 . Thomas, S.R. et al. (2002) J Biol Chem 277, 6017-24. |
Immunohistochemical analysis of paraffin-embedded HUVEC (left) and HeLa (right) cell pellets using eNOS (D8A6N) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using eNOS (D8A6N) Rabbit mAb. | |
colon: | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using eNOS (D8A6N) Rabbit mAb. | |
