| 货号 | 34648S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H Mk |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | Ajuba (D4D8P) Rabbit mAb recognizes endogenous levels of total Ajuba protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Ajuba belongs to the Zyxin/Ajuba family, members of which include LIMD1, LPP, Trip6 and Zyxin. Three LIM domains at the carboxy-terminus characterize this family of proteins. Zyxin/Ajuba family members associate with the actin cytoskeleton, are components of both cell-cell junction adhesive complex and integrin-mediated adhesive complex, and shuttle in and out of the nucleus (1). Ajuba is involved in Rac activation during cell migration and activates Aurora A in order to commit to mitosis (2-3). Ajuba and its family members also have an amino-terminal proline-rich pre-LIM region, and Ajuba augments ERK activation in a Ras-dependent manner (4). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 55 |
| 参考文献 | 1 . Feng, Y. and Longmore, G.D. (2005) Mol. Cell Biol. 25, 4010-4022. 2 . Pratt, S. J. et al. (2005) J. Cell Biol. 168, 813-824. 3 . Hirota, T. et al. (2003) Cell 114, 585-598. 4 . Goyal, R. et al. (1999) Mol. Cell Biol. 19, 4379-4389. |
Western blot analysis of extracts from various cell lines using Ajuba (D4D8P) Rabbit mAb. | |
Immunoprecipitation of Ajuba protein from PANC-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb XP® Isotype Control #3900, and lane 3 is Ajuba (D4D8P) Rabbit mAb. Western blot analysis was performed using Ajuba (D4D8P) Rabbit mAb. |
