| 货号 | 27281S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H M |
| 来源宿主 | Rabbit IgG |
| 应用 | W |
| 目标/特异性 | RNAse L (D4B4J) Rabbit mAb recognizes endogenous levels of total RNase L protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | RNase L is an antiviral protein that is expressed in most mammalian cells (1). Latent RNase L in the cytoplasm is activated by the second messenger 2’,5’-linked oligoadenylate (2-5A), which is produced by oligoadenylate synthase (OAS) after it binds viral double-stranded RNA (dsRNA) (2, 3). RNase L forms a crossed homodimer that is stabilized by kinase homology and ankyrin domains, which position two kinase extension nuclease domains for RNA recognition (4). RNase L then degrades both viral and cellular RNA (5). In mouse models, RNase L has been shown to produce small self-RNAs that act to amplify innate antiviral immunity through IFN-β induction (6). Research has also shown that RNase L forms a complex with Filamin A that acts as a barrier to restrict virus entry, and that RNase L can induce autophagy in response to viral infection (7, 8). Finally, research suggests RNase L may contribute to type I diabetes onset through immune response regulation (9). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 80 |
| 参考文献 | 1 . Zhou, A. et al. (2005) J Interferon Cytokine Res 25, 595-603. 2 . Hornung, V. et al. (2014) Nat Rev Immunol 14, 521-8. 3 . Floyd-Smith, G. et al. (1981) Science 212, 1030-2. 4 . Han, Y. et al. (2014) Science 343, 1244-8. 5 . Dong, B. and Silverman, R.H. (1997) J Biol Chem 272, 22236-42. 6 . Malathi, K. et al. (2007) Nature 448, 816-9. 7 . Malathi, K. et al. (2014) MBio 5, e02012. 8 . Siddiqui, M.A. and Malathi, K. (2012) J Biol Chem 287, 43651-64. 9 . Zeng, C. et al. (2014) J Endocrinol 223, 277-87. |
Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human RNase L (hRNase L-Myc/DDK; +), using RNase L (D4B4J) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using RNase L (D4B4J) Rabbit mAb. |
