| 货号 | 99199S |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | Human |
| 来源宿主 | Mouse IgG1 |
| 应用 | W IP IF-IC |
| 目标/特异性 | FoxO3a (D7D3Y) Mouse mAb detects exogenous and endogenous levels of total FoxO3a protein. The antibody does not detect the exogenously expressed family members FoxO1 or FoxO4. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9). |
| 存放说明 | -20C |
| 计算分子量 | 82 to 97 |
| 参考文献 | 1 . Anderson, M.J. et al. (1998) Genomics 47, 187-99. 2 . Galili, N. et al. (1993) Nat Genet 5, 230-5. 3 . Borkhardt, A. et al. (1997) Oncogene 14, 195-202. 4 . Nakae, J. et al. (1999) J Biol Chem 274, 15982-5. 5 . Rena, G. et al. (1999) J Biol Chem 274, 17179-83. 6 . Guo, S. et al. (1999) J Biol Chem 274, 17184-92. 7 . Seoane, J. et al. (2004) Cell 117, 211-23. 8 . Arden, K.C. (2004) Mol Cell 14, 416-8. 9 . Yang, Y. et al. (2005) EMBO J 24, 1021-32. |
Confocal immunofluorescent analysis of PC-3 cells, serum-starved overnight (left) or serum-starved overnight and treated with LY294002 #9901 (50 μM, 1 hr; right), using FoxO3a (D7D3Y) Mouse mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). | |
Immunoprecipitation of FoxO3a from 293T cell extracts. Lane 1 is 10% input, lane 2 is Mouse (G3A1) mAb Isotype Control #5415, and lane 3 is FoxO3a (D7D3Y) Mouse mAb. Western blot was performed using FoxO3a (D7D3Y) Mouse mAb. | |
Western blot analysis of extracts from 293T, MRK-nu-1, and PC-3 cells using FoxO3a (D7D3Y) Mouse mAb (upper) and β-Tubulin (D2N5G) Rabbit mAb #15115 (lower). |
