| 货号 | 97630S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W |
| 目标/特异性 | TSLP (D7U1X) Rabbit mAb recognizes endogenous levels of total TSLP protein. This antibody recognizes a 70 kDa protein of unknown identity. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TSLP (thymic stromal lymphopoietin) is a cytokine produced by epithelial cells at barrier surfaces and granulocytes (1). TSLP acts on dendritic cells, lymphocytes, and granulocytes by binding to a heterodimer composed of TSLPR (TSLP receptor) and IL-7Rα (2-5). Constitutive expression of TSLP at barrier surfaces contributes to homeostasis by promoting generation of noninflammatory dendritic cells (6). TSLP promotes the differentiation of Th2 cells and type 2 immune responses both in response to helminth infection and in the context of atopic diseases including asthma and atopic dermatitis (7-13). |
| 存放说明 | -20C |
| 计算分子量 | 19, 22 |
| 参考文献 | 1 . Soumelis, V. et al. (2002) Nat Immunol 3, 673-80. 2 . Reche, P.A. et al. (2001) J Immunol 167, 336-43. 3 . Levin, S.D. et al. (1999) J Immunol 162, 677-83. 4 . Park, L.S. et al. (2000) J Exp Med 192, 659-70. 5 . Pandey, A. et al. (2000) Nat Immunol 1, 59-64. 6 . Iliev, I.D. et al. (2007) J Exp Med 204, 2253-7. 7 . Taylor, B.C. et al. (2009) J Exp Med 206, 655-67. 8 . Ramalingam, T.R. et al. (2009) J Immunol 182, 6452-9. 9 . Soumelis, V. et al. (2002) Nat Immunol 3, 673-80. 10 . Ying, S. et al. (2005) J Immunol 174, 8183-90. 11 . Yoo, J. et al. (2005) J Exp Med 202, 541-9. 12 . Zhou, B. et al. (2005) Nat Immunol 6, 1047-53. 13 . Al-Shami, A. et al. (2005) J Exp Med 202, 829-39. |
Western blot analysis of recombinant human TSLP protein (hTSLP) using TSLP (D7U1X) Rabbit mAb. | |
Western blot analysis of extracts from NHBE (normal human bronchial epithelial cells), untransfected (-) or transfected with poly(I:C) (10 μg/ml, 7 hr; +), using TSLP (D7U1X) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Brefeldin A #9972 was added to cells at 300 ng/ml one hour after the start of the transfection. |
