| 货号 | 93815S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IF-F |
| 目标/特异性 | CB1 Receptor (D5N5C) Rabbit mAb recognizes endogenous levels of total CB1 Receptor protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The endocannabinoid system consists of the cannabinoid receptors, CB1 and CB2 receptors, the enzymes that produce and degrade the endogenous cannabinoid ligands (such as FAAH, DAG lipases, and MAG lipase), and the endocannabinoid ligands derived from the metabolism of arachidonic acid, 2-arachidonoylglycerol (2-AG) and anandamide (1-3). CB1 receptor belongs to the superfamily of G protein-coupled receptors (GPCRs) and harbors a large N-terminal extracellular domain, seven transmembrane domains, and a C-terminal intracellular tail. CB1 receptor is coupled to the Gai/o subunit of the G protein which inhibits adenylyl cyclases and regulates calcium and potassium ion channels (4). CB1 receptor is one of the most abundant GPCRs in the central nervous system. It has been show to play critical roles in the wiring of the brain during development (5), in neuronal plasticity (6), analgesia, drug abuse and metabolic homeostasis (7). In addition, CB1 receptor has been shown to interact with other GPCRs, to give rise to novel pharmacological and signaling heteromers with implication in diseases (8,9). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
| 参考文献 | 1 . Devane, W.A. et al. (1992) Science 258, 1946-9. 2 . Hanus, L. et al. (2001) Proc Natl Acad Sci U S A 98, 3662-5. 3 . Rozenfeld, R. (2011) Traffic 12, 12-8. 4 . Mackie, K. (2006) Annu Rev Pharmacol Toxicol 46, 101-22. 5 . Harkany, T. et al. (2008) Curr Opin Neurobiol 18, 338-45. 6 . Mackie, K. (2008) Mol Cell Endocrinol 286, S60-5. 7 . Mackie, K. (2006) Annu Rev Pharmacol Toxicol 46, 101-22. 8 . Rozenfeld, R. et al. (2011) EMBO J 30, 2350-63. 9 . Rozenfeld, R. et al. (2012) PLoS One 7, e29239. |
Immunoprecipitation of CB1 Receptor from mouse brain extracts. Lane 1 is 10 % input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CB1 Receptor (D5N5C) Rabbit mAb. Western blot analysis was performed using CB1 Receptor (D5N5C) Rabbit mAb. | |
Western blot analysis of extracts from various tissues using CB1 Receptor (D5N5C) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Confocal immunofluorescent analysis of mouse hippocampus: dentate gyrus (left), CA1 region (middle), and cerebellum (right), using CB1 Receptor (D5N5C) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
