| 货号 | 92043S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | TRIM21 (D1O1D) Rabbit mAb recognizes endogenous levels of total TRIM21 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Tripartite motif-containing protein 21 (TRIM21) is a cytosolic antiviral protein that is induced by interferons and acts as a cytosolic IgG receptor (1,2). TRIM21 is an activator of the innate immune system and targets antibody bound virions to the proteasome via its E3 ubiquitin ligase activity, leading to degradation of the virions in the cytosol (3,4). Interestingly, TRIM21 has also been shown to negatively regulate pathogen-induced interferon production through the polyubiquitin-mediated degradation of multiple targets including IRF3, IRF7, and DDX41 (5,6,7). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
| 参考文献 | 1 . Keeble, A.H. et al. (2008) Proc Natl Acad Sci U S A 105, 6045-50. 2 . Sjöstrand, M. et al. (2013) J Immunol 191, 3753-63. 3 . McEwan, W.A. et al. (2013) Nat Immunol 14, 327-36. 4 . Mallery, D.L. et al. (2010) Proc Natl Acad Sci U S A 107, 19985-90. 5 . Higgs, R. et al. (2008) J Immunol 181, 1780-6. 6 . Young, J.A. et al. (2011) J Biol Chem 286, 6521-31. 7 . Zhang, Z. et al. (2013) Nat Immunol 14, 172-8. |
Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing full-length human TRIM21 (hTRIM21; +), using TRIM21 (D1O1D) Rabbit mAb. | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Human Interferon-α1 (hIFN-α1) #8927 (10 ng/ml, 16 hr; +), using TRIM21 (D1O1D) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
