| 货号 | 91327S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Moneky |
| 来源宿主 | Rabbit IgG |
| 应用 | W |
| 目标/特异性 | hnRNP C1/C2 (D6S3N) Rabbit mAb recognizes endogenous levels of total hnRNP C1/C2 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Heterogeneous nuclear ribonucleoprotein C1/C2 (hnRNP C1/C2) has multiple biological functions including transcriptional regulation, DNA repair, and RNA processing. hnRNP C1/C2 acts as a ‘molecular ruler’ in the mRNA processing pathway, committing nascent transcripts from the chromatin template to the mRNA export pathway once the nascent transcript becomes longer than 200-300 nucleotides (1). hnRNP C1/C2 associates with SWI/SNF and NurD family members to form the locus control region (LCR)-associated remodeling complex (LARC), which binds to β-globin gene promoter to prevent transcriptional silencing. Studies indicate that without hnRNP C1/C2, LARC does not associate with its target DNA sequence (2,3). hnRNP C1/C2 and other hnRNP family members interact with DNA damage response (DDR) proteins (4). hnRNP proteins regulate double stranded break (DSB) repair by promoting either homologous recombination (HR) or non-homologous end joining (NHEJ) (4). hnRNP C1/C2 downregulates the expression of miR-21, which leads to the increased expression of programmed cell death 4 (PDCD4) protein in glioblastoma multiforme (GBM) (5). Research studies have shown that silencing of hnRNP C1/C2 renders GBM cells more susceptible to apoptosis (5). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
| 参考文献 | 1 . McCloskey, A. et al. (2012) Science 335, 1643-6. 2 . Huang, L. et al. (2011) Mol Cell Biol 31, 3472-84. 3 . Mahajan, M.C. et al. (2005) Proc Natl Acad Sci U S A 102, 15012-7. 4 . Haley, B. et al. (2009) Int J Radiat Biol 85, 643-55. 5 . Park, Y.M. et al. (2012) Mol Cell Biol 32, 4237-44. |
Western blot analysis of extracts from various cell lines using hnRNP C1/C2 (D6S3N) Rabbit mAb. |
