| 货号 | 91138S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W IF-IC |
| 目标/特异性 | Lamellipodin (D8A2K) Rabbit mAb recognizes endogenous levels of total lamellipodin protein. Based on the amino acid sequence of the immunogenic peptide, this antibody is expected to detect the canonical isoform (RMO1-RAPH1) only. |
| 使用方法 | WB(1:1000) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Regulation of actin dynamics at the leading edge of the cell drives cell adhesion, migration, and invasion. These processes are crucial for normal development, immune cell function, angiogenesis and cell division, and are dysregulated in diseases such as metastatic cancer (1, 2). |
| 存放说明 | -20C |
| 计算分子量 | 195 |
| 参考文献 | 1 . Rottner, K. and Stradal, T.E. (2011) Curr Opin Cell Biol 23, 569-78. 2 . Wells, A. et al. (2013) Trends Pharmacol Sci 34, 283-9. 3 . Hansen, S.D. and Mullins, R.D. (2015) Elife 4, . 4 . Law, A.L. et al. (2013) J Cell Biol 203, 673-89. 5 . Vehlow, A. et al. (2013) EMBO J 32, 2722-34. 6 . Lagarrigue, F. et al. (2015) Nat Commun 6, 8492. |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with full length human lamellipodin/Myc-DDK (+), using Lamellipodin (D8A2K) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower). | |
Western blot analysis of extracts from MCF7 cells, tranfected with control (-) or Lamellipodin siRNA (+), using Lamellipodin (D8A2K) Rabbit mAb (upper) or α-Actinin (D6F6) Rabbit mAb #6487. | |
Western blot analysis of extracts from various cell lines using Lamellipodin (D8A2K) Rabbit mAb. | |
Confocal immunofluorescent analysis of SNB-19 cells using Lamellipodin (D8A2K) Rabbit mAb (green). Actin filaments were labeled with DyLight® 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
