| 货号 | 37879S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat |
| 应用 | WB,IP |
| 目标/特异性 | Thrombospondin-1 (D7E5F) Rabbit mAb recognizes endogenous levels of total thrombospondin-1 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The adhesive glycoprotein thrombospondin-1 (THBS1, TSP1) localizes to the extracellular matrix (ECM) and mediates interactions between cells and the ECM and among cells. Thrombospondin-1 is a multi-domain, glycosylated protein that interacts with a wide variety of extracellular targets, including matrix metalloproteinases (MMPs), collagens, cell receptors, growth factors, and cytokines (1). The protein structure of THBS1 includes an amino-terminal laminin G-like domain, a von Willebrand factor-binding domain, and multiple thrombospondin (TSP) repeated sequences designated as type I, type II, or type III repeats. Each thrombospondin domain interacts with a distinct type of cell surface ligands or protein targets. The amino-terminal domain interacts with aggrecan, heparin, and integrin proteins. Type I TSP repeats interact with MMPs and CD36, while carboxy-terminal repeats bind the thrombospondin receptor CD47 (1). Through these interactions, THBS1 exerts diverse effects on different signaling pathways, such as VEGF receptor/NO signaling, TGFβ signaling, and the NF-κB pathway (2-5). Thrombospondin-1 is an important regulator of many biological processes, including cell adhesion/migration, apoptosis, angiogenesis, inflammation, vascular function, and cancer development (2-5). The activity of thrombospondin-1 is mainly regulated by extracellular proteases. The metalloproteinase ADAMTS1 cleaves thrombospondin, resulting in the release of peptides with anti-angiogenic properties. Elastase and plasmin proteases degrade the THBS1 protein and down regulate its activity (6). As THBS1 is an important protein inhibitor of angiogenesis, the development of thrombospondin-based compounds and their use in therapeutic studies may provide a beneficial approach to the treatment of cancer (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 170 |
| 参考文献 | 1 . Resovi, A. et al. (2014) Matrix Biol 37, 83-91. 2 . Lawler, P.R. and Lawler, J. (2012) Cold Spring Harb Perspect Med 2, a006627. 3 . Lopez-Dee, Z. et al. (2011) Mediators Inflamm 2011, 296069. 4 . Roberts, D.D. et al. (2012) Matrix Biol 31, 162-9. 5 . Kazerounian, S. et al. (2008) Cell Mol Life Sci 65, 700-12. 6 . Iruela-Arispe, M.L. (2008) Curr Drug Targets 9, 863-8. 7 . Mirochnik, Y. et al. (2008) Curr Drug Targets 9, 851-62. 8 . Taraboletti, G. et al. (2010) Oncotarget 1, 662-73. |
Western blot analysis of extracts from various cell lines using Thrombospondin-1 (D7E5F) Rabbit mAb. | |
Immunoprecipitation of thrombospondin-1 protein from ACHN cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Thrombospondin-1 (D7E5F) Rabbit mAb. Western blot analysis was performed using Thrombospondin-1 (D7E5F) Rabbit mAb. |
