| 货号 | 37705S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse |
| 应用 | WB,IP |
| 目标/特异性 | MLKL (D6W1K) Rabbit mAb (Mouse Specific) recognizes endogenous levels of total mouse MLKL protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Necroptosis, a regulated pathway for necrotic cell death, is triggered by a number of inflammatory signals including cytokines in the tumor necrosis factor (TNF) family, pathogen sensors such as toll-like receptors (TLRs), and ischemic injury (1,2). The process is negatively regulated by caspases and is initiated through a complex containing the RIP1 and RIP3 kinases, typically referred to as the necrosome. Mixed lineage kinase domain-like protein (MLKL) is a pseudokinase that was identified as downstream target of RIP3 in the necroptosis pathway (3,4). During necroptosis RIP3 is phosphorylated at Ser227, which recruits MLKL and leads to its phosphorylation at Thr357 and Ser358 (3). Knockdown of MLKL through multiple mechanisms results in inhibition of necroptosis (3-5). While the precise mechanism for MLKL-induced necroptosis is unclear, some studies have shown that necroptosis leads to oligomerization of MLKL and translocation to the plasma membrane, where it effects membrane integrity (6-9). |
| 存放说明 | -20C |
| 计算分子量 | 54 |
| 参考文献 | 1 . Christofferson, D.E. and Yuan, J. (2010) Curr Opin Cell Biol 22, 263-8. 2 . Kaczmarek, A. et al. (2013) Immunity 38, 209-23. 3 . Sun, L. et al. (2012) Cell 148, 213-27. 4 . Wang, Z. et al. (2012) Cell 148, 228-43. 5 . Wu, J. et al. (2013) Cell Res 23, 994-1006. 6 . Cai, Z. et al. (2014) Nat Cell Biol 16, 55-65. 7 . Chen, X. et al. (2014) Cell Res 24, 105-21. 8 . Wang, H. et al. (2014) Mol Cell 54, 133-46. 9 . Dondelinger, Y. et al. (2014) Cell Rep 7, 971-81. |
Western blot analysis of extracts from various cell lines using MLKL (D6W1K) Rabbit mAb (Mouse Specific). | |
Immunoprecipitation of MLKL protein from BaF3 cells. Lane 1 is 10% input, lane 2 is immunoprecipitated with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MLKL (D6W1K) Rabbit mAb (Mouse Specific). Western blot was performed with MLKL (D6W1K) Rabbit mAb (Mouse Specific). |
