| 货号 | 50996S |
| 同种亚型 | Mouse IgG2a |
| 反应种属 | Human, Mouse, Rat |
| 应用 | WB,IP |
| 目标/特异性 | Jak1 (D1T6W) Mouse mAb recognizes endogenous levels of total Jak1 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phosphotyrosine-binding domains. Activation of Jak kinases upon cytokine receptor binding is associated with tyrosine phosphorylation within their activation loops, including Tyr1034/1035 of Jak1, Tyr1007/1008 of Jak2, Tyr980/981 of Jak3, and Tyr1054/1055 of Tyk2. Many studies have indicated that various cytokine receptors have clear preferences that utilize distinct Jak family members. Aberrant regulation of Jak signaling is associated with a number of diseases, including myeloproliferative neoplasms, leukemia, and inflammatory disease (6). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
| 参考文献 | 1 . Leonard, W.J. and OShea, J.J. (1998) Annu Rev Immunol 16, 293-322. 2 . Darnell, J.E. (1997) Science 277, 1630-5. 3 . VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93. 4 . Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92. 5 . Zhu, T. et al. (1998) J Biol Chem 273, 10682-9. 6 . Babon, J.J. et al. (2014) Biochem J 462, 1-13. |
Western blot analysis of extracts from various cell lines using Jak1 (D1T6W) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of Jak1 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is immunoprecipitated with Mouse (G3A1) mAb IgG1 Isotype Control #5415, and lane 3 is Jak1 (D1T6W) Mouse mAb. Western blot was performed using Jak1 (D1T6W) Mouse mAb. |
