| 货号 | 50565S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat, Monkey |
| 应用 | WB,IHC,IF,F |
| 目标/特异性 | LRF/Pokemon (D7U2O) Rabbit mAb recognizes endogenous levels of total LRF/Pokemon protein. This antibody cross-reacts with a 44 kDa protein of unknown origin. |
| 使用方法 | Western Blotting (1:1000) Immunohistochemistry (Paraffin) (1:50) Immunofluorescence (Immunocytochemistry) (1:100) Flow Cytometry (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Zinc finger and BTB domain-containing protein 7A (LRF, Pokemon, FBI1) is a transcriptional repressor encoded by the ZBTB7A gene that belongs to the POK (POZ and Kruppel)/ZBTB (zinc finger and BTB) family (1). LRF is broadly expressed with elevated expression in a variety of cancers relative to normal tissues, including non-small cell lung cancer, breast cancer, ovarian cancer, prostate cancer, and hepatocellular carcinoma (1-8). Research studies suggest that LRF acts as an oncogene through various mechanisms including repression of the tumor suppressors ARF and Rb, and repression of the cell cycle arrest factor p21Cip1 (9-11). The LRF transcription factor plays key roles during several stages of hematopoiesis including promoting lymphoid progenitor cells to commit to B cell differentiation by repressing T cell-promoting Notch signals, and promoting cell survival during terminal erythroid differentiation through suppression of the proapoptotic factor Bim (12,13). |
| 存放说明 | -20C |
| 计算分子量 | 75 |
| 参考文献 | 1 . Lee, S.U. and Maeda, T. (2012) Immunol Rev 247, 107-19. 2 . Apostolopoulou, K. et al. (2007) J Pathol 213, 294-302. 3 . Zhao, Z.H. et al. (2008) Lung Cancer 62, 113-9. 4 . Qu, H. et al. (2010) Cancer Invest 28, 672-8. 5 . Aggarwal, A. et al. (2010) Exp Mol Pathol 89, 140-8. 6 . Jiang, L. et al. (2010) Mol Cancer 9, 318. 7 . Aggarwal, H. et al. (2011) Exp Mol Pathol 90, 226-30. 8 . Fang, F. et al. (2012) Cancer 118, 134-46. 9 . Maeda, T. et al. (2005) Nature 433, 278-85. 10 . Jeon, B.N. et al. (2008) J Biol Chem 283, 33199-210. 11 . Choi, W.I. et al. (2009) J Biol Chem 284, 12633-44. 12 . Maeda, T. et al. (2007) Science 316, 860-6. 13 . Maeda, T. et al. (2009) Dev Cell 17, 527-40. |
Western blot analysis of extracts from various cell lines using LRF/Pokemon (D7U2O) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using LRF/Pokemon (D7U2O) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human non-Hodgkins lymphoma using LRF/Pokemon (D7U2O) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using LRF/Pokemon (D7U2O) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse small intestine using LRF/Pokemon (D7U2O) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human tonsil using LRF/Pokemon (D7U2O) Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa cells, mock transfected (left, positive) or transfected with SignalSilence® LRF/Pokemon siRNA I #13350 (100 nM, 48 hr; right, negative), using LRF/Pokemon (D7U2O) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). | |
Confocal immunofluorescent analysis of HCT 116 cells using LRF/Pokemon (D7U2O) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). | |
Flow cytometric analysis of HeLa cells, transfected with SignalSilence® LRF/Pokemon siRNA I #13350 (100 nM, 48 hr; blue) or mock transfected (green), using LRF/Pokemon (D7U2O) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
