| 货号 | 50311S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat |
| 应用 | WB,IF |
| 目标/特异性 | EPLIN (D1A7A) Rabbit mAb recognizes endogenous levels of total EPLIN protein, including EPLIN-α and EPLIN-β isoforms. Based on the absence of signal in NCI-H28 cells, which appear to express low levels of β-Eplin, this antibody may have a preference for α-Eplin by immunofluorescence. |
| 使用方法 | Western Blotting (1:1000) Immunofluorescence (Immunocytochemistry) (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Epithelial Protein Lost in Neoplasm (EPLIN) is an actin-binding protein that regulates actin filament dynamics and cross-linking (1). Alpha and beta isoforms are generated from alternate promoters, with the EPLIN-β isoform representing the full-length protein and the EPLIN-α isoform lacking the amino-terminal 160 amino acids (2). Increased expression of EPLIN protein results in more abundant and larger actin stress fibers due to stabilizing of cross-links and inhibition of actin depolymerization. EPLIN protein inhibits Rac1-promoted membrane ruffling and Arp2/3-associated actin filament branching (1). Research studies demonstrate reduced EPLIN-α expression in tumor tissues, and correlate this reduction with increased invasiveness and poor clinical outcomes (3). The EPLIN protein is an important negative regulator of the epithelial-mesenchymal transition (EMT)(4). While EMT is a critical process during normal embryonic development, dysregulation in transformed cells is a key step in the transition to metastasis (5). |
| 存放说明 | -20C |
| 计算分子量 | 85, 110 |
| 参考文献 | 1 . Maul, R.S. et al. (2003) J Cell Biol 160, 399-407. 2 . Chen, S. et al. (2000) Gene 248, 69-76. 3 . Liu, Y. et al. (2012) Anticancer Res 32, 1283-9. 4 . Zhang, S. et al. (2011) Oncogene 30, 4941-52. 5 . Tsai, J.H. and Yang, J. (2013) Genes Dev 27, 2192-206. |
Western blot analysis of extracts from MDA-MB-231 and MDA-MB-486 cells using EPLIN (D1A7A) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from various cell lines using Eplin (D1A7A) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Confocal immunofluorescent analysis of T-47D cells (left, EPLIN-β high-expressing, EPLIN-α low-expressing), MRC-5 cells (center, EPLIN-β/EPLIN-α moderate-expressing) and NCI-H28 cells (right, EPLIN-β moderate-expressing, EPLIN-α low-expressing) using EPLIN (D1A7A) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
