| 货号 | 49661S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat |
| 应用 | IHC,IF |
| 目标/特异性 | TBR1 (D6C6X) Rabbit mAb recognizes endogenous levels of total TBR1 protein. Low levels of nuclear staining of unknown specificity have been observed in mouse small intestine, spleen, and pancreas. |
| 使用方法 | Immunohistochemistry (Paraffin) (1:250) Immunofluorescence (Frozen) (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous (IHC-P, IF-F), Transfected (W) |
| 背景 | T-box, brain, 1 (TBR1) is a transcription factor important in vertebrate embryo development. As a member of T-Box family of transcription factors, TBR1 is expressed in postmitotic glutamatergic projection neurons (1). During cortical neurogenesis, sequential expression of transcription factors Pax6, TBR2, and TBR1 regulates discrete steps in projection neuron differentiation (2). TBR1 is enriched in layer 6 of the developing cortex. In the absence of TBR1, TBR1 mutants exhibit profound defects in frontal cortex and layer 6 differentiation, suggesting that TBR1 regulates regional and laminar identity of postmitotic cortical neurons (3). Therefore, TBR1 expression can be used as a marker for postmitotic glutamatergic neurons and cortical laminar specificity. T-box, brain, 1 (TBR1) is a transcription factor important in vertebrate embryo development. As a member of T-Box family of transcription factors, TBR1 is expressed in postmitotic glutamatergic projection neurons (1). During cortical neurogenesis, sequential expression of transcription factors Pax6, TBR2, and TBR1 regulates discrete steps in projection neuron differentiation (2). TBR1 is enriched in layer 6 of the developing cortex. In the absence of TBR1, TBR1 mutants exhibit profound defects in frontal cortex and layer 6 differentiation, suggesting that TBR1 regulates regional and laminar identity of postmitotic cortical neurons (3). Therefore, TBR1 expression can be used as a marker for postmitotic glutamatergic neurons and cortical laminar specificity. |
| 存放说明 | -20C |
| 计算分子量 | 74 |
| 参考文献 | 1 . Hevner, R.F. et al. (2001) Neuron 29, 353-66. 2 . Englund, C. et al. (2005) J Neurosci 25, 247-51. 3 . Bedogni, F. et al. (2010) Proc Natl Acad Sci U S A 107, 13129-34. |
Immunohistochemical analysis of paraffin-embedded human brain using TBR1 (D6C6X) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse brain using TBR1 (D6C6X) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded E13 mouse embryo using TBR1 (D6C6X) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded rat brain, cortex (left) and cerebellum (right), using TBR1 (D6C6X) Rabbit mAb. | |
Confocal immunofluorescent analysis of E14.5 mouse cortex using TBR1 (D6C6X) Rabbit mAb (green) and GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) #3656 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
