| 货号 | 48938S |
| 同种亚型 | Mouse IgG2a |
| 反应种属 | Human, Mouse, Rat |
| 应用 | WB,IP,IF |
| 目标/特异性 | α-Smooth Muscle Actin (1A4) Mouse mAb (IF Formulated) recognizes endogenous levels of total α-smooth muscle actin protein |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:200) Immunofluorescence (Frozen) (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β- and γ-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility (1). The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells (1). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
| 参考文献 | 1 . Herman, I.M. (1993) Curr Opin Cell Biol 5, 48-55. |
Western blot analysis of extracts from various tissues using α-Smooth Muscle Actin (1A4) Mouse mAb (IF Formulated) (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, skeletal muscle samples are negative for α-smooth muscle actin. | |
Confocal immunofluorescent analysis of mouse small intestine (left) or skeletal muscle (right) using α-Smooth Muscle Actin (1A4) Mouse mAb (IF Formulated) (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
