| 货号 | 47411S |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | Human, Monkey |
| 应用 | WB,IP,IHC,IF,F |
| 目标/特异性 | Glucocorticoid Receptor (D4X9S) Mouse mAb recognizes endogenous levels of total glucocorticoid receptor protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:100) Immunohistochemistry (Paraffin) (1:300) Immunofluorescence (Immunocytochemistry) (1:800) Flow Cytometry (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Glucocorticoid hormones control cellular proliferation, inflammation, and metabolism through their association with the glucocorticoid receptor (GR)/NR3C1, a member of the nuclear hormone receptor superfamily of transcription factors (1). GR is composed of several conserved structural elements, including a carboxy-terminal ligand-binding domain (which also contains residues critical for receptor dimerization and hormone-dependent gene transactivation), a neighboring hinge region containing nuclear localization signals, a central zinc-finger-containing DNA-binding domain, and an amino-terminal variable region that participates in ligand-independent gene transcription. In the absence of hormone, a significant population of GR is localized to the cytoplasm in an inactive form via its association with regulatory chaperone proteins, such as HSP90, HSP70, and FKBP52. On hormone binding, GR is released from the chaperone complex and translocates to the nucleus as a dimer to associate with specific DNA sequences termed glucocorticoid response elements (GREs), thereby enhancing or repressing transcription of specific target genes (2). It was demonstrated that GR-mediated transcriptional activation is modulated by phosphorylation (3-5). Although GR can be basally phosphorylated in the absence of hormone, it becomes hyperphosphorylated upon binding receptor agonists. It has been suggested that hormone-dependent phosphorylation of GR may determine target promoter specificity, cofactor interaction, strength and duration of receptor signaling, receptor stability, and receptor subcellular localization (3). |
| 存放说明 | -20C |
| 计算分子量 | 80, 91, 94 |
| 参考文献 | 1 . Yamamoto, K.R. (1985) Annu. Rev. Genet 19, 209-252. 2 . Necela, B.M. and Cidlowski, J.A. (2003) Trends Pharmacol. Sci. 24, 58-61. 3 . Wang, Z. et al. (2002) J. Biol. Chem. 277, 26573-26580. 4 . Rogatsky, I. et al. (1998) J. Biol. Chem. 273, 14315-14321. 5 . Krstic, M. D. et al. (1997) Mol. Cell. Biol. 17, 3947-3954. |
Western blot analysis of extracts from various cell lines using Glucocorticoid Receptor (D4X9S) Mouse mAb. | |
Immunoprecipitation of glucocorticoid receptor from A549 cell extracts. Lane 1 is 10% input, lane 2 is Mouse (G3A1) mAb IgG1 Isotype Control #5415, lane 3 is Glucocorticoid Receptor (D4X9S) Mouse mAb. Western blot analysis was performed using Glucocorticoid Receptor (D4X9S) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Glucocorticoid Receptor (D4X9S) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Glucocorticoid Receptor (D4X9S) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, untreated (left) or treated with Dexamethasone #14776 (right), using Glucocorticoid Receptor (D4X9S) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Glucocorticoid Receptor (D4X9S) Mouse mAb. | |
Confocal immunofluorescent analysis of A549 cells, grown in serum free media for 1 d and either untreated (left) or treated with Dexamethasone #14776 (100 nM, 2 hr; right), using Glucocorticoid Receptor (D4X9S) Mouse mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054. | |
Flow cytometric analysis of Jurkat cells using Glucocorticoid Receptor (D4X9S) Mouse mAb (solid line) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (dashed line). Anti-mouse IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody. |
