| 货号 | 61994S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat |
| 应用 | WB,IP |
| 目标/特异性 | SIGMAR1 (D4J2E) Rabbit mAb recognizes endogenous levels of total SIGMAR1 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Sigma non-opioid intracellular receptor 1 (SIGMAR1) is an endoplasmic reticulum (ER) membrane chaperone that forms raft-like microdomains on the ER, where it interacts with mitochondria at the mitochondria-associated ER membrane domain (MAM). At MAM, SIGMAR1 maintains proper ER-mitochondrion Ca2+ signaling, regulates mitochondria function, and enhances cellular survival upon ER stress (1-4). When activated, SIGMAR1 translocates to ER and plasma membrane, where it interacts with a plethora of membrane proteins, including ion channels, neurotransmitter receptors, and kinases. SIGMAR1 also modulates a variety of neuronal functions, such as neuronal excitability, neuroplasticity, neuroprotection, and neurorestoration (5-7). SIGMAR1 binds to many anti-psychotic drugs and it is implicated in addiction, pain, neurodegenerative diseases, and depression (8-11). Recently, mutations in the SIGMAR1 gene have been reported to be associated with amyotrophic lateral sclerosis (12,13). Besides its important roles in central nervous system and peripheral nervous system, SIGMAR1 also enhances cancer cell migration and invasion (14,15). |
| 存放说明 | -20C |
| 计算分子量 | 25 |
| 参考文献 | 1 . Hanner, M. et al. (1996) Proc Natl Acad Sci U S A 93, 8072-7. 2 . Hayashi, T. and Su, T.P. (2003) J Pharmacol Exp Ther 306, 718-25. 3 . Hayashi, T. and Su, T.P. (2007) Cell 131, 596-610. 4 . Mori, T. et al. (2013) PLoS One 8, e76941. 5 . Griesmaier, E. et al. (2012) Exp Neurol 237, 388-95. 6 . Kourrich, S. et al. (2012) Trends Neurosci 35, 762-71. 7 . Liu, L.L. et al. (2016) Neuroscience 332, 53-60. 8 . Miyatake, R. et al. (2004) Biol Psychiatry 55, 85-90. 9 . Choi, S.R. et al. (2017) J Pain 18, 415-427. 10 . Huang, Y. et al. (2011) Curr Alzheimer Res 8, 765-70. 11 . Mandelli, L. et al. (2017) Adv Ther 34, 713-724. 12 . Fukunaga, K. et al. (2015) J Pharmacol Sci 127, 36-41. 13 . Al-Saif, A. et al. (2011) Ann Neurol 70, 913-9. 14 . Gueguinou, M. et al. (2017) Oncogene 36, 3640-7. 15 . Crottès, D. et al. (2016) Cancer Res 76, 607-18. |
Western blot analysis of extracts from BT-549, C2C12, and C6 cells using SIGMAR1 (D4J2E) Rabbit mAb. |
