| 货号 | 59036S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | IHC |
| 目标/特异性 | OX40L (D6K7R) Rabbit mAb recognizes endogenous levels of total OX40L protein. Staining of unknown specificity has been observed in skeletal muscle. |
| 使用方法 | IHC-Leica® Bond™ (1:200) Immunohistochemistry (Paraffin) (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | OX40 (TNFRSF4, CD134) is a member of the tumor necrosis factor (TNF) receptor superfamily that regulates T cell activity and immune responses. The OX40 protein contains four cysteine rich domains, a transmembrane domain, and a cytoplasmic tail containing a QEE motif (1,2). OX40 is primarily expressed on activated CD4+ and CD8+ T-cells, while the OX40 ligand (OX40L, TNFSF4, CD252) is predominantly expressed on activated antigen presenting cells (3-7). The engagement of OX40 with OX40L leads to the recruitment of TNF receptor-associated factors (TRAFs) and results in the formation of a TCR-independent signaling complex. One component of this complex, PKCθ, activates the NF-κB pathway (2,8). OX40 signaling through Akt can also enhance TCR signaling directly (9). Research studies indicate that the OX40L-OX40 pathway is associated with inflammation and autoimmune diseases (10). Additional research studies show that OX40 agonists augment anti-tumor immunity in several cancer types (11,12). |
| 存放说明 | -20C |
| 参考文献 | 1 . Croft, M. (2010) Annu Rev Immunol 28, 57-78. 2 . So, T. and Croft, M. (2012) Front Immunol 3, 133. 3 . Paterson, D.J. et al. (1987) Mol Immunol 24, 1281-90. 4 . Mallett, S. et al. (1990) EMBO J 9, 1063-8. 5 . Dürkop, H. et al. (1995) Br J Haematol 91, 927-31. 6 . Godfrey, W.R. et al. (1994) J Exp Med 180, 757-62. 7 . Al-Shamkhani, A. et al. (1997) J Biol Chem 272, 5275-82. 8 . So, T. et al. (2011) Proc Natl Acad Sci U S A 108, 2903-8. 9 . So, T. and Croft, M. (2013) Front Immunol 4, 139. 10 . Gough, M.J. and Weinberg, A.D. (2009) Adv Exp Med Biol 647, 94-107. 11 . Weinberg, A.D. et al. (2011) Immunol Rev 244, 218-31. 12 . Linch, S.N. et al. (2015) Front Oncol 5, 34. |
Immunohistochemical analysis of paraffin-embedded human ovarian clear cell adenocarcinoma using OX40L (D6K7R) Rabbit mAb (IHC Specific). Analysis was performed on the Leica® Bond™ Rx. | |
Immunohistochemical analysis of paraffin-embedded HUVEC cell pellet (left, positive) or MCF7 cell pellet (right, negative) using OX40L (D6K7R) Rabbit mAb (IHC Specific). | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using OX40L (D6K7R) Rabbit mAb (IHC Specific). | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using OX40L (D6K7R) Rabbit mAb (IHC Specific). | |
Immunohistochemical analysis of paraffin-embedded human tonsil using OX40L (D6K7R) Rabbit mAb (IHC Specific). |
