| 货号 | 15571S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IHC-P |
| 目标/特异性 | TFF1/pS2 (D2Y1J) Rabbit mAb recognizes endogenous levels of total TFF1/pS2 protein. This antibody does not cross-react with either TFF2 or TFF3 proteins. |
| 使用方法 | W IHC-P |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The trefoil factor (TFF) family of proteins (TFF1/pS2, TFF2, and TFF3) are a group of highly conserved, secreted polypeptides that are expressed by mucus-secreting cells of the gastrointestinal tract. Within the gastrointestinal tract, TFFs display both common and distinct expression patterns (1). Collectively, the TFF family of proteins play a prominant role in the protection and repair of the mucous epithelia lining the gastrointestinal tract through their interactions with mucins (2). TFFs have been shown to regulate a number of cellular processes such as migration, apoptosis, and proliferation. In humans, dysregulated expression of TFFs has been observed in inflammatory bowel diseases as well as tumors of the breast, colon, lung, and stomach (2). |
| 存放说明 | -20C |
| 计算分子量 | 13 |
| 参考文献 | 1 . Madsen, J. et al. (2007) J Histochem Cytochem 55, 505-13. 2 . Taupin, D. and Podolsky, D.K. (2003) Nat Rev Mol Cell Biol 4, 721-32. 3 . Mashimo, H. et al. (1996) Science 274, 262-5. 4 . Lefebvre, O. et al. (1996) Science 274, 259-62. 5 . May, F.E. and Westley, B.R. (2015) Endocr Relat Cancer 22, 465-79. 6 . Corte, M.D. et al. (2006) Breast Cancer Res Treat 96, 63-72. 7 . Taupin, D. et al. (2001) Lab Invest 81, 397-408. 8 . Carvalho, R. et al. (2002) Lab Invest 82, 1319-26. 9 . Katoh, M. (2003) Int J Mol Med 12, 3-9. 10 . McChesney, P.A. et al. (2006) Cancer Res 66, 1346-53. |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using TFF1/pS2 (D2Y1J) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded KATO III (left) and MDA-MB-231 (right) cell pellets using TFF1/pS2 (D2Y1J) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TFF1/pS2 (D2Y1J) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using TFF1/pS2 (D2Y1J) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Note that MDA-MB-231 cells are deficient in TFF1 protein expression. | |
Western blot analysis of extracts from normal human stomach, liver, and kidney using TFF1/pS2 (D2Y1J) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Normal human liver and kidney tissues are reported to be deficient in TFF1/pS2 protein expression (1). | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human TFF1 protein (hTFF1-Myc/DDK; +), Myc/DDK-tagged full-length human TFF2 protein (hTFF2-Myc/DDK; +) and Myc/DDK-tagged full-length human TFF3 protein (hTFF3-Myc/DDK; +), using TFF1/pS2 (D2Y1J) Rabbit mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower). |
