| 货号 | 99940S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse |
| 应用 | WB,IHC |
| 目标/特异性 | CD3ε (D4V8L) Rabbit mAb recognizes endogenous levels of total mouse CD3ε protein. Non-specific staining in mouse pancreas has been observed. |
| 使用方法 | Western Blotting (1:1000) IHC-Leica® Bond™ (1:100) Immunohistochemistry (Paraffin) (1:150) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck (5). |
| 存放说明 | -20C |
| 计算分子量 | 22 |
| 参考文献 | 1 . Kuhns, M.S. et al. (2006) Immunity 24, 133-9. 2 . Pitcher, L.A. and van Oers, N.S. (2003) Trends Immunol 24, 554-60. 3 . Osman, N. et al. (1996) Eur. J. Immunol. 26, 1063-1068. 4 . Hatada, M.H. et al. (1995) Nature 377, 32-38. 5 . Gil, D. et al. (2002) Cell 109, 901-912. |
Western blot analysis of extracts from mouse spleen, EL4 cells, and Raji cells using CD3ε (D4V8L) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD3ε (D4V8L) Rabbit mAb performed on the Leica® BOND™ Rx. | |
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD3ε (D4V8L) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD3ε (D4V8L) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse lung using CD3ε (D4V8L) Rabbit mAb. | |
Multiplex immunohistochemical analysis of paraffin-embedded mouse LL2 syngeneic tumor tissue using CD3ε (D4V8L) rabbit mAb (red), F4/80 (D2S9R) XP® rabbit mAb #70076 (orange), PD-1 (D7D5W) XP® rabbit mAb #84651 (green), PD-L1 (D5V3B) rabbit mAb #64988 (yellow), CD8α (D4W2Z) XP® rabbit mAb #98941 (magenta) and pan keratin (C11) mouse mAb #4279 (cyan). |
